Pyrimidine salvage enzymes in entamoeba histolytica

الملخص EN

Extracts of Entamoeba histolytica were surveyed for the enzymes which catalyse pryimidine salvage.

Cytidine deaminase (EC.

3.5.4.5), uridine kinase (EC.

2.7.1.48), cytidine kinase (EC, 2.71.43) and thymidine Kinase (EC.

2,7.1.75) were all detected at relatively high activities, whereas, cytosine deaminase (EC.

3.5.4.1) and anabolic phos-phorylase were not detected.

Catabolic phosphoiylase (EC.

2.4.2.3) was found to be active only on uridine.

Uracil phosphoribosyl transferase (EC.

2.4.2.9) also was detected but, only at low activity.

The results suggest that in E.

histolytica the main route of pyrimidine nucleotide synthesis is through the action of kinases.