In vitro callus formation from pomegranate (Punica granatum L.)‎ shoot tips

الملخص EN

Calluswas initiated from cultured shoot lips (I em in length) of pomegranate i Punicagranatum L.) cv.

"Banat" on a basal medium containing Murashige and Skoog (MS) salts, vitamins.

glycine , i-inositol , sucrose, agar and IAA/BA at concentrations of00/00,0.1/1 .0.0.3/3.0.0,5/5 .0 and 1.0 / 10.0 rng/L .

Callus growth was betterin light than in darkness .

Also.

callus formation occurred on MS medium containing benzylarninopurine (SA)atconcentrations of0,2.5,5and 7.5mgIL.

The best callus growth was in the medium containing 5 rng / L BA.

When shoottips were cultured on MS medium supplemented with O.

2,4 and 6 rng/L NAA.

callus was formed and then subcultured twice .

The formed callus was maintained for 105 days.