In vitro culture and propagation of Punica granatum L.

الملخص EN

Shoot tip cxplarus (I em in length) of pomegranate (Punica granatum L.) proliferated and produced shoots after 8 weeks of in vitro culture.

Shoot tip explants were cultured on Murashige and Skoog (MS) basal medium supplemented with a combinations of IAA : BA at ranges of 0.0-1 .3 Rng / l and 0.0-3.0 mg / l respectively.

Shoot multiplication occurred efficiently 4 weeks after the initial growth was recultured .

BA alone (O.R mg / l produced the highest proliferative effect though BA + IAA at 3.0:0 .2 mgll had a relatively high multiplication effect (3.4 shoot / explant) .

The interaction of IAA and BA showed to have an effect on shoot length and shoot number per culture.

The produced shoots were subculture on half strength MS medium supplemented with 2.0 mgi1 IBA and 0.25 gil activated charcoal.

The percentage of motel! cultures was 100 %.