Molecular detection of cyla and investigation of heamolysin type in enterococcus faecalis

الملخص EN

In this study, 39 isolates of Enterococcus faecalis were collected from different source (stool, urine and vagina).

It was seen that 79.4 % of the isolates are non–hemolyticon human blood agar, where20.6 % were hemolytic.

Since,5.1 % revealedá-hemolysis and 15.5 % â-hemolysis.

All â-hemolytic isolates were from urine and vagina samples but not from stool samples.

Moreover, investigation for cytolysinA (cylA) by using specific primer and PCR technique was showed that all isolates from different sources gave positive amplicons with cylA primer.

cytolysinA may be part from Hly activity in Enterococcus faecalis.