Somatic embryo mediated mass production of Catharanthus roseus in culture vessel (bioreactor) : a comparative study
المؤلفون المشاركون
Mujib, A.
Ali, Muzamil
Isah, Tasiu
Dipti
المصدر
Saudi Journal of Biological Sciences
العدد
المجلد 21، العدد 5 (30 نوفمبر/تشرين الثاني 2014)، ص ص. 442-449، 8ص.
الناشر
تاريخ النشر
2014-11-30
دولة النشر
السعودية
عدد الصفحات
8
التخصصات الرئيسية
الموضوعات
الملخص EN
The purpose of this study was to evaluate and compare the use of liquid and solid Murashige and Skoog (MS) medium in different culture vessels for mass production of Catharanthus roseus, an important source of anticancerous compounds, vincristine and vinblastine.
Three media conditions i.e.
agar-solidified medium (S), liquid medium in agitated conical flask (L) and growtek bioreactor (B) were used.
Rapid propagation was achieved through in vitro somatic embryogenesis pathway.
The process of embryogenesis has been categorized into induction, proliferation, maturation and germination stages.
All in vitro embryogenesis stages were conducted by withdrawing spent liquid medium and by adding fresh MS medium.
In optimized 4.52 lM 2,4-D added MS, the callus biomass growth was low in solid (1.65 g) compared to liquid medium in agitated conical flask (1.95 g) and in bioreactor (2.11 g).
The number of normal somatic embryos was more in solid medium (99.75/50 mg of callus mass) compared to liquid medium used in conical flask (83.25/callus mass) and growtek bioreactor (84.88/callus mass).
The in vitro raised embryos maturated in GA3 (2.60 lM) added medium; and in bioreactor the embryo growth was high, a maximum length of 9.82 mm was observed at the end of four weeks.
These embryos germinated into seedlings in BAP (2.22 lM) added medium and the embryo germination ability was more (59.41%) in bioreactor compared to liquid medium in conical flask (55.5%).
Shoot length (11.25 mm) was also high in bioreactor compared to agitated conical flask.
The liquid medium used in agitated conical flask and bioreactor increased seedling production efficiency, at the same time it also reduced plant recovery time.
The embryo generated plants grew normally in outdoor conditions.
The exploitation of medium to large culture vessel or bioreactor may make the process more efficient in getting large number of Catharanthus plant as it is the only source of anti-cancerous alkaloids, vincristine and vinblastine.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Mujib, A.& Ali, Muzamil& Isah, Tasiu& Dipti. 2014. Somatic embryo mediated mass production of Catharanthus roseus in culture vessel (bioreactor) : a comparative study. Saudi Journal of Biological Sciences،Vol. 21, no. 5, pp.442-449.
https://search.emarefa.net/detail/BIM-409978
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ali, Muzamil…[et al.]. Somatic embryo mediated mass production of Catharanthus roseus in culture vessel (bioreactor) : a comparative study. Saudi Journal of Biological Sciences Vol. 21, no. 5 (Nov. 2014), pp.442-449.
https://search.emarefa.net/detail/BIM-409978
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Mujib, A.& Ali, Muzamil& Isah, Tasiu& Dipti. Somatic embryo mediated mass production of Catharanthus roseus in culture vessel (bioreactor) : a comparative study. Saudi Journal of Biological Sciences. 2014. Vol. 21, no. 5, pp.442-449.
https://search.emarefa.net/detail/BIM-409978
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 448-449
رقم السجل
BIM-409978
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر