Development, optimization and validation of HPLC method for determination of febuxostat using design of experiments (DoE)
مقدم أطروحة جامعية
مشرف أطروحة جامعية
الجامعة
جامعة عمان الأهلية
الكلية
كلية الصيدلة و العلوم الطبية
القسم الأكاديمي
قسم العلوم الصيدلانية
دولة الجامعة
الأردن
الدرجة العلمية
ماجستير
تاريخ الدرجة العلمية
2014
الملخص الإنجليزي
The purpose of this study was to develop a new high performance liquid chromatographic for determination of febuxostat in tablet dosage form.
In the present study a rapid, simple, economical HPLC-PDA procedure has been developed and validated.
The HPLC method was developed and optimised using Box-Behnken-design (BBD), after studying the physicochemical properties of febuxostat (solubility, melting point, 1R, UV).
The Optimized mobile phase was [34% buffer (250|il TEA/L), 33% acelonitrile and 33% methanol , the pi I value was adjusted to 3.0 10.10] using orthophosphoric acid.
The flow rate was 1.0 ml/min, the signals were monitored using PDA detector (set between A 200-400 nm)using windows based LC solution software quantitation was carried out at wave length of 315 nm.
The linearity range of the method was from 5 to 50 p.g/ml for febuxostat.
LOD was 0.3201 pg/ml for febuxostat, whereas LOQ was 0.9702|ig/ml.
The precision of the method was less than 2% which indicates highly precise result during the study.
I he accuracy of the method were ranged from 99.39%-l 00.97%.
There were two minor degradation products of febuxostat after exposure to hydrogen peroxide (35%) with retention time 2.34 and 2.66 while exposure to alkaline stress resulted 5 different minor degraded products.
No degradation products of febuxostat were observed after exposure to UV light.
In conclusion the method presented here is sensitive, rapid, accurate, precise, economic, robust, rugged and selective for the routine analysis of the drug in formulation (tablet) and raw material.
التخصصات الرئيسية
الموضوعات
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Baradan, Majid Y.. (2014). Development, optimization and validation of HPLC method for determination of febuxostat using design of experiments (DoE). (Master's theses Theses and Dissertations Master). Al-Ahliyya Amman University, Jordan
https://search.emarefa.net/detail/BIM-412353
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Baradan, Majid Y.. Development, optimization and validation of HPLC method for determination of febuxostat using design of experiments (DoE). (Master's theses Theses and Dissertations Master). Al-Ahliyya Amman University. (2014).
https://search.emarefa.net/detail/BIM-412353
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Baradan, Majid Y.. (2014). Development, optimization and validation of HPLC method for determination of febuxostat using design of experiments (DoE). (Master's theses Theses and Dissertations Master). Al-Ahliyya Amman University, Jordan
https://search.emarefa.net/detail/BIM-412353
لغة النص
الإنجليزية
نوع البيانات
رسائل جامعية
رقم السجل
BIM-412353
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر