Cloning of the recombinant cytochrome p450 cyp141 protein of mycobacterium tuberculosis as a diagnostic target and vaccine candidate
المؤلفون المشاركون
Mumtaz, Hasan
Rabii Faradonbeh, Muhammad
Darban Sarokhalil, Davood
Feizabadi, Muhammad Mehdi
Alvandi, Amirhooshang
Sulaymani, Nada
Gholipour, Abu al-Fadl
المصدر
Iranian Red Crescent Medical Journal
العدد
المجلد 16، العدد 11 (30 نوفمبر/تشرين الثاني 2014)، ص ص. 1-5، 5ص.
الناشر
تاريخ النشر
2014-11-30
دولة النشر
الإمارات العربية المتحدة
عدد الصفحات
5
التخصصات الرئيسية
الموضوعات
الملخص EN
Background : Tuberculosis has been announced as a global emergency by World Health Organization and the second infectious agent of mortality worldwide.
The general policy in the development of new vaccines is to develop some vaccines with higher efficiency not only for infants but also for adults compared with the Bacillus Calmette-Guerin vaccine.
Recently, cytochrome P450 cyp141 has been introduced as a new target for detecting Mycobacterium tuberculosis from clinical samples.
Objectives : The aim of this study was to clone this gene in order to pave the way for more evaluation.
Materials and Methods : M.
tuberculosis H37Rv DNA was extracted by a standard phenol-chlorophorm protocol.
After designing the specific primers, P450 cyp141 gene was replicated by PCR.
The purified PCR products were then subcloned into the pTZ57R / T plasmid vector.
After extraction, enzyme digestion, and recombinant pTZ57R / T-cyp141 plasmid vector sequencing, the aforementioned products were cloned into a pET-26b plasmid vector.
Then, the recombinant pET26b-cyp141 plasmid molecules were transformed to Escherichia coli strain BL21 (DE3) using the transformation method.
Next, the recombinant pET26b-cyp141 plasmids were purified and evaluated by the enzyme digestion analysis.
Results : The cloning of P450 cyp141 gene was confirmed by the enzyme digestion and sequencing of the recombinant pTZ57R / T-cyp141 and pET26b-cyp141 plasmid vectors.
Conclusions : The results of this study demonstrated that the P450 cyp141 gene was successfully cloned into a pET26b plasmid vector as an expression vector.
In this paper, for the first time in Iran, this gene was cloned for more purposes, including the expression and purification of the recombinant cytochrome P450 cyp141 protein.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Rabii Faradonbeh, Muhammad& Darban Sarokhalil, Davood& Feizabadi, Muhammad Mehdi& Alvandi, Amirhooshang& Mumtaz, Hasan& Sulaymani, Nada…[et al.]. 2014. Cloning of the recombinant cytochrome p450 cyp141 protein of mycobacterium tuberculosis as a diagnostic target and vaccine candidate. Iranian Red Crescent Medical Journal،Vol. 16, no. 11, pp.1-5.
https://search.emarefa.net/detail/BIM-428183
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Rabii Faradonbeh, Muhammad…[et al.]. Cloning of the recombinant cytochrome p450 cyp141 protein of mycobacterium tuberculosis as a diagnostic target and vaccine candidate. Iranian Red Crescent Medical Journal Vol. 16, no. 11 (Nov. 2014), pp.1-5.
https://search.emarefa.net/detail/BIM-428183
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Rabii Faradonbeh, Muhammad& Darban Sarokhalil, Davood& Feizabadi, Muhammad Mehdi& Alvandi, Amirhooshang& Mumtaz, Hasan& Sulaymani, Nada…[et al.]. Cloning of the recombinant cytochrome p450 cyp141 protein of mycobacterium tuberculosis as a diagnostic target and vaccine candidate. Iranian Red Crescent Medical Journal. 2014. Vol. 16, no. 11, pp.1-5.
https://search.emarefa.net/detail/BIM-428183
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 5
رقم السجل
BIM-428183
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر