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Normalized real-time PCR for diagnosis of H. pylori infection
المؤلفون المشاركون
al-Shamahi, Hasan Abd al-Wahhab
al-Muayyad, Ibtisam E.
al-Ghalibi, Sayyid M.
Nashir, Akram T.
al-Habashi, Nizar N.
المصدر
العدد
المجلد 23، العدد 2 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-17، 17ص.
الناشر
تاريخ النشر
2014-12-31
دولة النشر
قطر
عدد الصفحات
17
التخصصات الرئيسية
الموضوعات
الملخص EN
Objectives : There is increasing interest in the use of quantitative PCR (q-PCR) for diagnosis of H.
pylori infection.
However, the assay remains largely unstandardized, making comparison between studies unreliable.
The objective of this study was to assess accuracy of a normalized q-PCR assay for diagnosis of the infection.
Subjects and methods : Seventy-six fresh gastric biopsy specimens were collected from patients undergoing upper gastrointestinal tract endoscopy and examined by rapid urease test (RUT), culture, and a commercial TaqMan q-PCR assay targeting the ureA gene.
Counts obtained from the latter assay were normalized to the human ACTB gene.
A subject was considered to be infected if two or more assays were positive.
Results : The detection rates were 42.1 %, 52.6 %, and 78.9 % by culture, RUT and q-PCR, respectively.
Bacterial density ranged 0.005 to 4800 bacteria per 100 human cells.
Because q-PCR showed low initial specificity (45.7 %), the cutoff value for the assay was recalculated as 1 bacterium 2 per 100 human cells, using ROC curve analysis.
Accordingly, the sensitivities and specificities were 79.5 % and 97.3 %, respectively, for culture ; 94.9 % and 91.9 %, respectively, for RUT ; and 94.9 % and 94.6 %, respectively, for q-PCR.
By gold standard, 39 of the dyspeptic patients (51.3 %) were found to be infected.
Conclusions : With the identified cutoff value, the q-PCR assay diagnosed H.
pylori infection with an accuracy slightly superior to that of RUT.
However, the possibility that low counts detected only by q-PCR represent true infections warrants further investigation.
Normalization of bacterial counts for standardization of q-PCR H.
pylori assays is recommended.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Muayyad, Ibtisam E.& al-Ghalibi, Sayyid M.& al-Shamahi, Hasan Abd al-Wahhab& Nashir, Akram T.& al-Habashi, Nizar N.. 2014. Normalized real-time PCR for diagnosis of H. pylori infection. Qatar Medical Journal،Vol. 23, no. 2, pp.1-17.
https://search.emarefa.net/detail/BIM-431505
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Muayyad, Ibtisam E.…[et al.]. Normalized real-time PCR for diagnosis of H. pylori infection. Qatar Medical Journal Vol. 23, no. 2 (2014), pp.1-17.
https://search.emarefa.net/detail/BIM-431505
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Muayyad, Ibtisam E.& al-Ghalibi, Sayyid M.& al-Shamahi, Hasan Abd al-Wahhab& Nashir, Akram T.& al-Habashi, Nizar N.. Normalized real-time PCR for diagnosis of H. pylori infection. Qatar Medical Journal. 2014. Vol. 23, no. 2, pp.1-17.
https://search.emarefa.net/detail/BIM-431505
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes appendices : p. 13-17
رقم السجل
BIM-431505
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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