Replication Past the γ-Radiation-Induced Guanine-Thymine Cross-Link G[8,5-Me]T by Human and Yeast DNA Polymerase η
المؤلفون المشاركون
Basu, Ashis K.
Raychaudhury, Paromita
المصدر
العدد
المجلد 2010، العدد 2010 (31 ديسمبر/كانون الأول 2010)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2010-09-13
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
γ-Radiation-induced intrastrand guanine-thymine cross-link, G[8,5-Me]T, hinders replication in vitro and is mutagenic in mammalian cells.
Herein we report in vitro translesion synthesis of G[8,5-Me]T by human and yeast DNA polymerase η (hPol η and yPol η).
dAMP misincorporation opposite the cross-linked G by yPol η was preferred over correct incorporation of dCMP, but further extension was 100-fold less efficient for G∗:A compared to G∗:C.
For hPol η, both incorporation and extension were more efficient with the correct nucleotides.
To evaluate translesion synthesis in the presence of all four dNTPs, we have developed a plasmid-based DNA sequencing assay, which showed that yPol η was more error-prone.
Mutational frequencies of yPol η and hPol η were 36% and 14%, respectively.
Targeted G→T was the dominant mutation by both DNA polymerases.
But yPol η induced targeted G→T in 23% frequency relative to 4% by hPol η.
For yPol η, targeted G→T and G→C constituted 83% of the mutations.
By contrast, with hPol η, semi-targeted mutations (7.2%), that is, mutations at bases near the lesion, occurred at equal frequency as the targeted mutations (6.9%).
The kind of mutations detected with hPol η showed significant similarities with the mutational spectrum of G[8,5-Me]T in human embryonic kidney cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Raychaudhury, Paromita& Basu, Ashis K.. 2010. Replication Past the γ-Radiation-Induced Guanine-Thymine Cross-Link G[8,5-Me]T by Human and Yeast DNA Polymerase η. Journal of Nucleic Acids،Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-446378
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Raychaudhury, Paromita& Basu, Ashis K.. Replication Past the γ-Radiation-Induced Guanine-Thymine Cross-Link G[8,5-Me]T by Human and Yeast DNA Polymerase η. Journal of Nucleic Acids No. 2010 (2010), pp.1-10.
https://search.emarefa.net/detail/BIM-446378
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Raychaudhury, Paromita& Basu, Ashis K.. Replication Past the γ-Radiation-Induced Guanine-Thymine Cross-Link G[8,5-Me]T by Human and Yeast DNA Polymerase η. Journal of Nucleic Acids. 2010. Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-446378
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-446378
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر