Quantification of Photocyanine in Human Serum by High-Performance Liquid Chromatography-Tandem Mass Spectrometry and Its Application in a Pharmacokinetic Study

الملخص EN

Photocyanine is a novel anticancer drug.

Its pharmacokinetic study in cancer patients is therefore very important for choosing doses, and dosing intervals in clinical application.

A rapid, selective and sensitive high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed and validated for the determination of photocyanine in patient serum.

Sample preparation involved one-step protein precipitation by adding methanol and N,N-dimethyl formamide to 0.1 mL serum.

The detection was performed on a triple quadrupole tandem mass spectrometer operating in multiple reaction-monitoring (MRM) mode.

Each sample was chromatographed within 7 min.

Linear calibration curves were obtained for photocyanine at a concentration range of 20–2000 ng/mL (r>0.995), with the lower limit of quantification (LLOQ) being 20 ng/mL.

The intrabatch accuracy ranged from 101.98% to 107.54%, and the interbatch accuracy varied from 100.52% to 105.62%.

Stability tests showed that photocyanine was stable throughout the analytical procedure.

This study is the first to utilize the HPLC-MS/MS method for the pharmacokinetic study of photocyanine in six cancer patients who had received a single dose of photocyanine (0.1 mg/kg) administered intravenously.