Quantification of Photocyanine in Human Serum by High-Performance Liquid Chromatography-Tandem Mass Spectrometry and Its Application in a Pharmacokinetic Study
المؤلفون المشاركون
Zhan, Jing
Liao, Hai
Zou, Ben-Yan
Bi, Bing-Tian
Li, Su
Deng, Li-Ting
Feng, Kun-Yao
المصدر
Journal of Analytical Methods in Chemistry
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-06-23
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Photocyanine is a novel anticancer drug.
Its pharmacokinetic study in cancer patients is therefore very important for choosing doses, and dosing intervals in clinical application.
A rapid, selective and sensitive high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed and validated for the determination of photocyanine in patient serum.
Sample preparation involved one-step protein precipitation by adding methanol and N,N-dimethyl formamide to 0.1 mL serum.
The detection was performed on a triple quadrupole tandem mass spectrometer operating in multiple reaction-monitoring (MRM) mode.
Each sample was chromatographed within 7 min.
Linear calibration curves were obtained for photocyanine at a concentration range of 20–2000 ng/mL (r>0.995), with the lower limit of quantification (LLOQ) being 20 ng/mL.
The intrabatch accuracy ranged from 101.98% to 107.54%, and the interbatch accuracy varied from 100.52% to 105.62%.
Stability tests showed that photocyanine was stable throughout the analytical procedure.
This study is the first to utilize the HPLC-MS/MS method for the pharmacokinetic study of photocyanine in six cancer patients who had received a single dose of photocyanine (0.1 mg/kg) administered intravenously.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Bi, Bing-Tian& Zou, Ben-Yan& Deng, Li-Ting& Zhan, Jing& Liao, Hai& Feng, Kun-Yao…[et al.]. 2014. Quantification of Photocyanine in Human Serum by High-Performance Liquid Chromatography-Tandem Mass Spectrometry and Its Application in a Pharmacokinetic Study. Journal of Analytical Methods in Chemistry،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-446468
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Bi, Bing-Tian…[et al.]. Quantification of Photocyanine in Human Serum by High-Performance Liquid Chromatography-Tandem Mass Spectrometry and Its Application in a Pharmacokinetic Study. Journal of Analytical Methods in Chemistry No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-446468
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Bi, Bing-Tian& Zou, Ben-Yan& Deng, Li-Ting& Zhan, Jing& Liao, Hai& Feng, Kun-Yao…[et al.]. Quantification of Photocyanine in Human Serum by High-Performance Liquid Chromatography-Tandem Mass Spectrometry and Its Application in a Pharmacokinetic Study. Journal of Analytical Methods in Chemistry. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-446468
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-446468
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر