Neuroprotective Effects of Hesperidin, a Plant Flavanone, on Rotenone-Induced Oxidative Stress and Apoptosis in a Cellular Model for Parkinson’s Disease
المؤلفون المشاركون
Karthikeyan, Subburayan
Essa, Musthafa Mohamed
Tamilselvam, Kuppusamy
Selvaraju, Subash
Manivasagam, Thamilarasan
Prasad, Nagarajan Rajendra
Guillemin, Gilles J.
Thenmozhi, Arokyasamy Justin
Braidy, Nady
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-09-24
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
العلوم الطبيعية والحياتية (متداخلة التخصصات)
الأحياء
الملخص EN
Rotenone a widely used pesticide that inhibits mitochondrial complex I has been used to investigate the pathobiology of PD both in vitro and in vivo.
Studies have shown that the neurotoxicity of rotenone may be related to its ability to generate reactive oxygen species (ROS), leading to neuronal apoptosis.
The current study was carried out to investigate the neuroprotective effects of hesperidin, a citrus fruit flavanol, against rotenone-induced apoptosis in human neuroblastoma SK-N-SH cells.
We assessed cell death, mitochondrial membrane potential, ROS generation, ATP levels, thiobarbituric acid reactive substances, reduced glutathione (GSH) levels, and the activity of catalase, superoxide dismutase (SOD) and glutathione peroxidase (GPx) using well established assays.
Apoptosis was determined in normal, rotenone, and hesperidin treated cells, by measuring the protein expression of cytochrome c (cyt c), caspases 3 and 9, Bax, and Bcl-2 using the standard western blotting technique.
The apoptosis in rotenone-induced SK-N-SH cells was accompanied by the loss of mitochondrial membrane potential, increased ROS generation, the depletion of GSH, enhanced activities of enzymatic antioxidants, upregulation of Bax, cyt c, and caspases 3 and 9, and downregulation of Bcl-2, which were attenuated in the presence of hesperidin.
Our data suggests that hesperidin exerts its neuroprotective effect against rotenone due to its antioxidant, maintenance of mitochondrial function, and antiapoptotic properties in a neuroblastoma cell line.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Tamilselvam, Kuppusamy& Braidy, Nady& Manivasagam, Thamilarasan& Essa, Musthafa Mohamed& Prasad, Nagarajan Rajendra& Karthikeyan, Subburayan…[et al.]. 2013. Neuroprotective Effects of Hesperidin, a Plant Flavanone, on Rotenone-Induced Oxidative Stress and Apoptosis in a Cellular Model for Parkinson’s Disease. Oxidative Medicine and Cellular Longevity،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-446499
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Tamilselvam, Kuppusamy…[et al.]. Neuroprotective Effects of Hesperidin, a Plant Flavanone, on Rotenone-Induced Oxidative Stress and Apoptosis in a Cellular Model for Parkinson’s Disease. Oxidative Medicine and Cellular Longevity No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-446499
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Tamilselvam, Kuppusamy& Braidy, Nady& Manivasagam, Thamilarasan& Essa, Musthafa Mohamed& Prasad, Nagarajan Rajendra& Karthikeyan, Subburayan…[et al.]. Neuroprotective Effects of Hesperidin, a Plant Flavanone, on Rotenone-Induced Oxidative Stress and Apoptosis in a Cellular Model for Parkinson’s Disease. Oxidative Medicine and Cellular Longevity. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-446499
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-446499
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر