Protection Effect of Endomorphins on Advanced Glycation End Products Induced Injury in Endothelial Cells
المؤلفون المشاركون
Tian, Limin
Guo, Qian
Quan, Jinxing
Zhang, Qi
Yan, Liping
Niu, Ruilan
Wei, Suhong
Liu, Jing
Liu, Hua
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-04-08
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Endomorphins (EMs) have a very important bridge-function in cardiovascular, endocrinological, and neurological systems.
This study is to investigate the effects of EMs on the synthesis and secretion of vasoactive substances induced by advanced glycation end products in primary cultured human umbilical vein endothelial cells (HUVECs).
Firstly, HUVECs were stimulated with AGEs-bovine serum albumin (AGEs-BSA), bovine serum albumin (BSA), or both AGEs-BSA and EMs together, respectively.
Then, HUVEC survival rate was calculated by MTT assay, the levels of NO, endothelial nitric oxide synthase (eNOS), and inducible nitric oxide synthase (iNOS) were detected by colorimetric analysis, and the contents of endothelin-1 (ET-1) were detected by ELISA.
The mRNA levels of eNOS and ET-1 were measured by RT-PCR.
The expression of p38 mitogen-activated protein kinase (p38 MAPK) was detected by immunofluorescence assay.
The results showed that the mRNA expression and secretion of eNOS were significantly enhanced after incubation with EMs compared to those with AGEs-BSA, while the secretion of NO and iNOS, mRNA expression, and secretion of ET-1 had opposite changes.
The fluorescence intensity of p38MAPK in nuclear was decreased after pretreatment with EMs compared to incubation with AGEs-BSA.
Conclusion.
The present study suggests that EMs have certain protection effect on AGEs-BSA-induced injury in HUVEC.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liu, Jing& Yan, Liping& Niu, Ruilan& Tian, Limin& Zhang, Qi& Quan, Jinxing…[et al.]. 2013. Protection Effect of Endomorphins on Advanced Glycation End Products Induced Injury in Endothelial Cells. Journal of Diabetes Research،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-446799
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liu, Jing…[et al.]. Protection Effect of Endomorphins on Advanced Glycation End Products Induced Injury in Endothelial Cells. Journal of Diabetes Research No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-446799
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liu, Jing& Yan, Liping& Niu, Ruilan& Tian, Limin& Zhang, Qi& Quan, Jinxing…[et al.]. Protection Effect of Endomorphins on Advanced Glycation End Products Induced Injury in Endothelial Cells. Journal of Diabetes Research. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-446799
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-446799
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر