S100b Counteracts Neurodegeneration of Rat Cholinergic Neurons in Brain Slices after Oxygen-Glucose Deprivation

الملخص EN

Alzheimer's disease is a severe chronic neurodegenerative disorder characterized by beta-amyloid plaques, tau pathology, cerebrovascular damage, inflammation, reactive gliosis, and cell death of cholinergic neurons.

The aim of the present study is to test whether the glia-derived molecule S100b can counteract neurodegeneration of cholinergic neurons after oxygen-glucose deprivation (OGD) in organotypic brain slices of basal nucleus of Meynert.

Our data showed that 3 days of OGD induced a marked decrease of cholinergic neurons (60% of control), which could be counteracted by 50 μg/mL recombinant S100b.

The effect was dose and time dependent.

Application of nerve growth factor or fibroblast growth factor-2 was less protective.

C-fos-like immunoreactivity was enhanced 3 hours after OGD indicating metabolic stress.

We conclude that S100b is a potent neuroprotective factor for cholinergic neurons during ischemic events.