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Proteomics Strategy for Identifying Candidate Bioactive Proteins in Complex Mixtures : Application to the Platelet Releasate
المؤلفون المشاركون
Cagney, Gerard
O'Connor, Roisin
O'Brien, Colm
de Stefani, Andreas
Fitzgerald, Desmond
Cryan, Lorna M.
Wynne, Kieran
المصدر
Journal of Biomedicine and Biotechnology
العدد
المجلد 2010، العدد 2010 (31 ديسمبر/كانون الأول 2010)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2010-03-24
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Proteomic approaches have proven powerful at identifying large numbers of proteins, but there are fewer reports of functional characterization of proteins in biological tissues.
Here, we describe an experimental approach that fractionates proteins released from human platelets, linking bioassay activity to identity.
We used consecutive orthogonal separation platforms to ensure sensitive detection: (a) ion-exchange of intact proteins, (b) SDS-PAGE separation of ion-exchange fractions and (c) HPLC separation of tryptic digests coupled to electrospray tandem mass spectrometry.
Migration of THP-1 monocytes in response to complete or fractionated platelet releasate was assessed and located to just one of the forty-nine ion-exchange fractions.
Over 300 proteins were identified in the releasate, with a wide range of annotated biophysical and biochemical properties, in particular platelet activation, adhesion, and wound healing.
The presence of PEDF and involucrin, two proteins not previously reported in platelet releasate, was confirmed by western blotting.
Proteins identified within the fraction with monocyte promigratory activity and not in other inactive fractions included vimentin, PEDF, and TIMP-1.
We conclude that this analytical platform is effective for the characterization of complex bioactive samples.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
O'Connor, Roisin& Cryan, Lorna M.& Wynne, Kieran& de Stefani, Andreas& Fitzgerald, Desmond& O'Brien, Colm…[et al.]. 2010. Proteomics Strategy for Identifying Candidate Bioactive Proteins in Complex Mixtures : Application to the Platelet Releasate. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-12.
https://search.emarefa.net/detail/BIM-447002
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
O'Connor, Roisin…[et al.]. Proteomics Strategy for Identifying Candidate Bioactive Proteins in Complex Mixtures : Application to the Platelet Releasate. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-12.
https://search.emarefa.net/detail/BIM-447002
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
O'Connor, Roisin& Cryan, Lorna M.& Wynne, Kieran& de Stefani, Andreas& Fitzgerald, Desmond& O'Brien, Colm…[et al.]. Proteomics Strategy for Identifying Candidate Bioactive Proteins in Complex Mixtures : Application to the Platelet Releasate. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-12.
https://search.emarefa.net/detail/BIM-447002
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-447002
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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