Altered Pulmonary Lymphatic Development in Infants with Chronic Lung Disease

الملخص EN

Pulmonary lymphatic development in chronic lung disease (CLD) has not been investigated, and anatomy of lymphatics in human infant lungs is not well defined.

Hypothesis.

Pulmonary lymphatic hypoplasia is present in CLD.

Method.

Autopsy lung tissues of eighteen subjects gestational ages 22 to 40 weeks with and without history of respiratory morbidity were stained with monoclonal antipodoplanin and reviewed under light microscopy.

Percentage of parenchyma podoplanin stained at the acinar level was determined using computerized image analysis; 9 CLD and 4 control subjects gestational ages 27 to 36 weeks were suitable for the analysis.

Results.

Distinct, lymphatic-specific staining with respect to other vascular structures was appreciated in all gestations.

Infants with and without respiratory morbidity had comparable lymphatic distribution which extended to the alveolar ductal level.

Podoplanin staining per parenchyma was increased and statistically significant in the CLD group versus controls at the alveolar ductal level (0.06% ± 0.02% versus 0.04% ± 0.01%, 95% CI −0.04% to −0.002%, P<0.03).

Conclusion.

Contrary to our hypothesis, the findings show that there is an increase in alveolar lymphatics in CLD.

It is suggested that the findings, by expanding current knowledge of CLD pathology, may offer insight into the development of more effective therapies to tackle CLD.