Experimental Study of Local Inner Ear Gene Therapy for Controlling Autoimmune Sensorineural Hearing Loss
المؤلفون المشاركون
Lu, Ling
Cai, Wen-jun
Tan, Chang-qiang
Huang, He
Qian, Xiao-yun
Gao, Xia
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-04-07
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
This study aimed to investigate the efficacy of gene therapy for treating autoimmune sensorineural hearing loss (ASHL) via local administration of a recombinant adenovirus vector containing the Fas ligand or interleukin IL-10 gene.
Guinea pigs were divided into four groups, with different microinjections in the scala tympani.
Group A were injected with FasL-EGFP, B with IL-10-EGFP, C with EGFP, and D with artificial perilymph.
Seven days later, auditory brain-stem response (ABR) was tested, and the temporal bone was stained and observed by light microscopy.
The spiral ligament and basement membrane were observed using transmission electron microscopy.
FasL and IL-10 expression were examined using immunofluorescence histochemistry.
Immunohistochemical analysis showed that the recombinant adenovirus vector in Groups A, B, and C can transfect the stria vascularis, the spiral ligament, the organ of Corti, the spiral ganglion, the region surrounding the small blood vessel in the modiolus, and the cochlear bone wall.
Compared with those in Groups C and D, the ABR wave III mean thresholds were significantly lower and the inner ear immunoinflammatory responses in Groups A and B were significantly alleviated.
Inhibition of immunoinflammatory response alleviated immunoinflammatory injury and auditory dysfunction.
This technique shows potential as a novel therapy for ASHL.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Tan, Chang-qiang& Gao, Xia& Cai, Wen-jun& Qian, Xiao-yun& Lu, Ling& Huang, He. 2014. Experimental Study of Local Inner Ear Gene Therapy for Controlling Autoimmune Sensorineural Hearing Loss. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-448390
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Tan, Chang-qiang…[et al.]. Experimental Study of Local Inner Ear Gene Therapy for Controlling Autoimmune Sensorineural Hearing Loss. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-448390
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Tan, Chang-qiang& Gao, Xia& Cai, Wen-jun& Qian, Xiao-yun& Lu, Ling& Huang, He. Experimental Study of Local Inner Ear Gene Therapy for Controlling Autoimmune Sensorineural Hearing Loss. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-448390
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-448390
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر