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Differential Gene Expression Profiling of Enriched Human Spermatogonia after Short- and Long-Term Culture
المؤلفون المشاركون
Azizi, Hossein
Conrad, Sabine
Skutella, Thomas
Hennenlotter, Jörg
Hatami, Maryam
Kubista, Mikael
Bonin, Michael
Renninger, Markus
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-17، 17ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-03-12
دولة النشر
مصر
عدد الصفحات
17
التخصصات الرئيسية
الملخص EN
This study aimed to provide a molecular signature for enriched adult human stem/progenitor spermatogonia during short-term (<2 weeks) and long-term culture (up to more than 14 months) in comparison to human testicular fibroblasts and human embryonic stem cells.
Human spermatogonia were isolated by CD49f magnetic activated cell sorting and collagen−/laminin+ matrix binding from primary testis cultures obtained from ten adult men.
For transcriptomic analysis, single spermatogonia-like cells were collected based on their morphology and dimensions using a micromanipulation system from the enriched germ cell cultures.
Immunocytochemical, RT-PCR and microarray analyses revealed that the analyzed populations of cells were distinct at the molecular level.
The germ- and pluripotency-associated genes and genes of differentiation/spermatogenesis pathway were highly expressed in enriched short-term cultured spermatogonia.
After long-term culture, a proportion of cells retained and aggravated the “spermatogonial” gene expression profile with the expression of germ and pluripotency-associated genes, while in the majority of long-term cultured cells this molecular profile, typical for the differentiation pathway, was reduced and more genes related to the extracellular matrix production and attachment were expressed.
The approach we provide here to study the molecular status of in vitro cultured spermatogonia may be important to optimize the culture conditions and to evaluate the germ cell plasticity in the future.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Conrad, Sabine& Azizi, Hossein& Hatami, Maryam& Kubista, Mikael& Bonin, Michael& Hennenlotter, Jörg…[et al.]. 2014. Differential Gene Expression Profiling of Enriched Human Spermatogonia after Short- and Long-Term Culture. BioMed Research International،Vol. 2014, no. 2014, pp.1-17.
https://search.emarefa.net/detail/BIM-448740
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Conrad, Sabine…[et al.]. Differential Gene Expression Profiling of Enriched Human Spermatogonia after Short- and Long-Term Culture. BioMed Research International No. 2014 (2014), pp.1-17.
https://search.emarefa.net/detail/BIM-448740
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Conrad, Sabine& Azizi, Hossein& Hatami, Maryam& Kubista, Mikael& Bonin, Michael& Hennenlotter, Jörg…[et al.]. Differential Gene Expression Profiling of Enriched Human Spermatogonia after Short- and Long-Term Culture. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-17.
https://search.emarefa.net/detail/BIM-448740
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-448740
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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