Human Periodontal Ligament Derived Progenitor Cells : Effect of STRO-1 Cell Sorting and Wnt3a Treatment on Cell Behavior
المؤلفون المشاركون
van den Beucken, Jeroen J. J. P.
Yang, Fang
Yan, Xiang-Zhen
Both, Sanne K.
Yang, Pi-Shan
Jansen, John A.
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-04-28
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Objectives.
STRO-1 positive periodontal ligament cells (PDLCs) and unsorted PDLCs have demonstrated potential for periodontal regeneration, but the comparison between unsorted cells and the expanded STRO-1 sorted cells has never been reported.
Additionally, Wnt3a is involved in cell proliferation thus may benefit in vitro PDLC expansion.
The aim was to evaluate the effect of STRO-1 cell sorting and Wnt3a treatment on cell behavior of human PDLCs (hPDLCs).
Materials and Methods.
STRO-1 positive hPDLCs were sorted and the sorted cells were expanded and compared with their unsorted parental cells.
Thereafter, hPDLCs were treated with or without Wnt3a and the cell proliferation, self-renewal, and osteogenic differentiation were evaluated.
Results.
No differences were measured between the expanded STRO-1-sorted cells and unsorted parental cells in terms of proliferation, CFU, and mineralization capacity.
Wnt3a enhanced the proliferation and self-renewal ability of hPDLCs significantly as displayed by higher DNA content values, a shorter cell population doubling time, and higher expression of the self-renewal gene Oct4.
Moreover, Wnt3a promoted the expansion of hPDLCs for 5 passages without affecting cell proliferation, CFU, and osteogenic capacity.
Conclusions.
Expanded STRO-1-sorted hPDLCs showed no superiority compared to their unsorted parental cells.
On the other hand, Wnt3a promotes the efficient hPDLC expansion and retains the self-renewal and osteogenic differentiation capacity.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yan, Xiang-Zhen& Both, Sanne K.& Yang, Pi-Shan& Jansen, John A.& van den Beucken, Jeroen J. J. P.& Yang, Fang. 2014. Human Periodontal Ligament Derived Progenitor Cells : Effect of STRO-1 Cell Sorting and Wnt3a Treatment on Cell Behavior. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-449312
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yan, Xiang-Zhen…[et al.]. Human Periodontal Ligament Derived Progenitor Cells : Effect of STRO-1 Cell Sorting and Wnt3a Treatment on Cell Behavior. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-449312
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yan, Xiang-Zhen& Both, Sanne K.& Yang, Pi-Shan& Jansen, John A.& van den Beucken, Jeroen J. J. P.& Yang, Fang. Human Periodontal Ligament Derived Progenitor Cells : Effect of STRO-1 Cell Sorting and Wnt3a Treatment on Cell Behavior. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-449312
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-449312
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر