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Human Periodontal Ligament Derived Progenitor Cells : Effect of STRO-1 Cell Sorting and Wnt3a Treatment on Cell Behavior
المؤلفون المشاركون
van den Beucken, Jeroen J. J. P.
Yang, Fang
Yan, Xiang-Zhen
Both, Sanne K.
Yang, Pi-Shan
Jansen, John A.
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-04-28
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Objectives.
STRO-1 positive periodontal ligament cells (PDLCs) and unsorted PDLCs have demonstrated potential for periodontal regeneration, but the comparison between unsorted cells and the expanded STRO-1 sorted cells has never been reported.
Additionally, Wnt3a is involved in cell proliferation thus may benefit in vitro PDLC expansion.
The aim was to evaluate the effect of STRO-1 cell sorting and Wnt3a treatment on cell behavior of human PDLCs (hPDLCs).
Materials and Methods.
STRO-1 positive hPDLCs were sorted and the sorted cells were expanded and compared with their unsorted parental cells.
Thereafter, hPDLCs were treated with or without Wnt3a and the cell proliferation, self-renewal, and osteogenic differentiation were evaluated.
Results.
No differences were measured between the expanded STRO-1-sorted cells and unsorted parental cells in terms of proliferation, CFU, and mineralization capacity.
Wnt3a enhanced the proliferation and self-renewal ability of hPDLCs significantly as displayed by higher DNA content values, a shorter cell population doubling time, and higher expression of the self-renewal gene Oct4.
Moreover, Wnt3a promoted the expansion of hPDLCs for 5 passages without affecting cell proliferation, CFU, and osteogenic capacity.
Conclusions.
Expanded STRO-1-sorted hPDLCs showed no superiority compared to their unsorted parental cells.
On the other hand, Wnt3a promotes the efficient hPDLC expansion and retains the self-renewal and osteogenic differentiation capacity.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yan, Xiang-Zhen& Both, Sanne K.& Yang, Pi-Shan& Jansen, John A.& van den Beucken, Jeroen J. J. P.& Yang, Fang. 2014. Human Periodontal Ligament Derived Progenitor Cells : Effect of STRO-1 Cell Sorting and Wnt3a Treatment on Cell Behavior. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-449312
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yan, Xiang-Zhen…[et al.]. Human Periodontal Ligament Derived Progenitor Cells : Effect of STRO-1 Cell Sorting and Wnt3a Treatment on Cell Behavior. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-449312
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yan, Xiang-Zhen& Both, Sanne K.& Yang, Pi-Shan& Jansen, John A.& van den Beucken, Jeroen J. J. P.& Yang, Fang. Human Periodontal Ligament Derived Progenitor Cells : Effect of STRO-1 Cell Sorting and Wnt3a Treatment on Cell Behavior. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-449312
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-449312
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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