Inhibition of Recombinant D-Amino Acid Oxidase from Trigonopsis variabilis by Salts

الملخص EN

Inhibition of recombinant D-amino acid oxidase from Trigonopsis variabilis (TvDAAO) activity in the presence of different sodium salts and potassium chloride is reported.

A competitive inhibition pattern by sodium chloride was observed, and an inhibition constant value of Ki=85 mM was calculated.

Direct connection of NaCl inhibition with FAD cofactor dissociation was confirmed by measuring the fluorescence of tryptophanyl residues of the holoenzyme.