Characterization of Multidrug Resistant ESBL-Producing Escherichia coli Isolates from Hospitals in Malaysia
المؤلفون المشاركون
Thong, Kwai-Lin
Yeo, Chew-Chieng
Puthucheary, Savithri
Yasin, Rohani
Lim, King-Ting
المصدر
Journal of Biomedicine and Biotechnology
العدد
المجلد 2009، العدد 2009 (31 ديسمبر/كانون الأول 2009)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2009-08-05
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
The emergence of Escherichia coli that produce extended spectrum β-lactamases (ESBLs) and are multidrug resistant (MDR) poses antibiotic management problems.
Forty-seven E.
coli isolates from various public hospitals in Malaysia were studied.
All isolates were sensitive to imipenem whereas 36 were MDR (resistant to 2 or more classes of antibiotics).
PCR detection using gene-specific primers showed that 87.5% of the ESBL-producing E.
coli harbored the blaTEM gene.
Other ESBL-encoding genes detected were blaOXA, blaSHV, and blaCTX-M.
Integron-encoded integrases were detected in 55.3% of isolates, with class 1 integron-encoded intI1 integrase being the majority.
Amplification and sequence analysis of the 5′CS region of the integrons showed known antibiotic resistance-encoding gene cassettes of various sizes that were inserted within the respective integrons.
Conjugation and transformation experiments indicated that some of the antibiotic resistance genes were likely plasmid-encoded and transmissible.
All 47 isolates were subtyped by PFGE and PCR-based fingerprinting using random amplified polymorphic DNA (RAPD), repetitive extragenic palindromes (REPs), and enterobacterial repetitive intergenic consensus (ERIC).
These isolates were very diverse and heterogeneous.
PFGE, ERIC, and REP-PCR methods were more discriminative than RAPD in subtyping the E.
coli isolates.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lim, King-Ting& Yasin, Rohani& Yeo, Chew-Chieng& Puthucheary, Savithri& Thong, Kwai-Lin. 2009. Characterization of Multidrug Resistant ESBL-Producing Escherichia coli Isolates from Hospitals in Malaysia. Journal of Biomedicine and Biotechnology،Vol. 2009, no. 2009, pp.1-10.
https://search.emarefa.net/detail/BIM-451128
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lim, King-Ting…[et al.]. Characterization of Multidrug Resistant ESBL-Producing Escherichia coli Isolates from Hospitals in Malaysia. Journal of Biomedicine and Biotechnology No. 2009 (2009), pp.1-10.
https://search.emarefa.net/detail/BIM-451128
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lim, King-Ting& Yasin, Rohani& Yeo, Chew-Chieng& Puthucheary, Savithri& Thong, Kwai-Lin. Characterization of Multidrug Resistant ESBL-Producing Escherichia coli Isolates from Hospitals in Malaysia. Journal of Biomedicine and Biotechnology. 2009. Vol. 2009, no. 2009, pp.1-10.
https://search.emarefa.net/detail/BIM-451128
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-451128
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر