A Novel Algorithm for the Assessment of Blood-Brain Barrier Permeability Suggests That Brain Topical Application of Endothelin-1 Does Not Cause Early Opening of the Barrier in Rats
المؤلفون المشاركون
Milakara, D.
Major, S.
Alam, M.
Friedman, A.
Dreier, J. P.
Kang, E. J.
Jorks, D.
المصدر
Cardiovascular Psychiatry and Neurology
العدد
المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2011-03-30
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
There are a number of different experimental methods for ex vivo assessment of blood-brain barrier (BBB) opening based on Evans blue dye extravasation.
However, these methods require many different steps to prepare the brain and need special equipment for quantification.
We here report a novel, simple, and fast semiquantitative algorithm to assess BBB integrity ex vivo.
The method is particularly suitable for cranial window experiments, since it keeps the spatial information about where the BBB opened.
We validated the algorithm using sham controls and the established model of brain topical application of the bile salt dehydrocholate for early BBB disruption.
We then studied spreading depolarizations in the presence and the absence of the vasoconstrictor endothelin-1 and found no evidence of early BBB opening (three-hour time window).
The algorithm can be used, for example, to assess BBB permeability ex vivo in combination with dynamic in vivo studies of BBB opening.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Jorks, D.& Milakara, D.& Alam, M.& Kang, E. J.& Major, S.& Friedman, A.…[et al.]. 2011. A Novel Algorithm for the Assessment of Blood-Brain Barrier Permeability Suggests That Brain Topical Application of Endothelin-1 Does Not Cause Early Opening of the Barrier in Rats. Cardiovascular Psychiatry and Neurology،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-451425
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Jorks, D.…[et al.]. A Novel Algorithm for the Assessment of Blood-Brain Barrier Permeability Suggests That Brain Topical Application of Endothelin-1 Does Not Cause Early Opening of the Barrier in Rats. Cardiovascular Psychiatry and Neurology No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-451425
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Jorks, D.& Milakara, D.& Alam, M.& Kang, E. J.& Major, S.& Friedman, A.…[et al.]. A Novel Algorithm for the Assessment of Blood-Brain Barrier Permeability Suggests That Brain Topical Application of Endothelin-1 Does Not Cause Early Opening of the Barrier in Rats. Cardiovascular Psychiatry and Neurology. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-451425
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-451425
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر