Phenotypic and Molecular Characterization of Plasmid Mediated AmpC β-Lactamases among Escherichia coli, Klebsiella spp., and Proteus mirabilis Isolated from Urinary Tract Infections in Egyptian Hospitals
المؤلفون المشاركون
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-06-09
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
The incidence of resistance by Enterobacteriaceae to β-lactam/β-lactamase inhibitors combination is increasing in Egypt.
Three phenotypic techniques, comprising AmpC disk diffusion and inhibition dependent methods using phenylboronic acid (PBA) and cloxacillin, were compared to PCR based method for detection of plasmid mediated AmpC β-lactamase in common urinary tract isolates.
A total of 143 isolates, including E.
coli, Klebsiella pneumonia, and Proteus mirabilis, were collected from urinary tract infections cases in Egyptian hospitals.
Plasmid encoded AmpC genes were detected by PCR in 88.46% of cefoxitin resistant isolates.
The most prevalent AmpC gene family was CIT including CMY-2, CMY-4, and two CMY-2 variants.
The second prevalent gene was DHA-1 which was detected in E.
coli and Klebsiella pneumonia.
The genes EBC, FOX, and MOX were also detected but in small percentage.
Some isolates were identified as having more than one pAmpC gene.
The overall sensitivity and specificity of phenotypic tests for detection of AmpC β-lactamase showed that AmpC disk diffusion and inhibition dependent method by cloxacillin were the most sensitive and the most specific disk tests.
PCR remains the gold standard for detection of AmpC β-lactamases.
This study represents the first report of CMY-2 variants of CMY-42 and CMY-102 β-lactamase-producing E.
coli, Klebsiella pneumonia, and Proteus mirabilis isolates in Egypt.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Helmy, Mai M.& Wasfi, Reham. 2014. Phenotypic and Molecular Characterization of Plasmid Mediated AmpC β-Lactamases among Escherichia coli, Klebsiella spp., and Proteus mirabilis Isolated from Urinary Tract Infections in Egyptian Hospitals. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-451582
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Helmy, Mai M.& Wasfi, Reham. Phenotypic and Molecular Characterization of Plasmid Mediated AmpC β-Lactamases among Escherichia coli, Klebsiella spp., and Proteus mirabilis Isolated from Urinary Tract Infections in Egyptian Hospitals. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-451582
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Helmy, Mai M.& Wasfi, Reham. Phenotypic and Molecular Characterization of Plasmid Mediated AmpC β-Lactamases among Escherichia coli, Klebsiella spp., and Proteus mirabilis Isolated from Urinary Tract Infections in Egyptian Hospitals. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-451582
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-451582
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر