Kinetic Analysis of Guanidine Hydrochloride Inactivation of β-Galactosidase in the Presence of Galactose
المؤلفون المشاركون
Nwamba, Charles O.
Chilaka, Ferdinand C.
المصدر
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-09-13
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Inactivation of purified β-Galactosidase was done with GdnHCl in the absence and presence of varying [galactose] at 50°C and at pH 4.5.
Lineweaver-Burk plots of initial velocity data, in the presence and absence of guanidine hydrochloride (GdnHCl) and galactose, were used to determine the relevant Km and Vmax values, with p-nitrophenyl β-D-galactopyranoside (pNPG) as substrate, S.
Plots of ln([P]∞−[P]t) against time in the presence of GdnHCl yielded the inactivation rate constant, A.
Plots of A versus [S] at different galactose concentrations were straight lines that became increasingly less steep as the [galactose] increased, showing that A was dependent on [S].
Slopes and intercepts of the 1/[P]∞ versus 1/[S] yielded k+0 and k'+0, the microscopic rate constants for the free enzyme and the enzyme-substrate complex, respectively.
Plots of k+0 and k'+0 versus [galactose] showed that galactose protected the free enzyme as well as the enzyme-substrate complex (only at the lowest and highest [galactose]) against GdnHCl inactivation.
In the absence of galactose, GdnHCl exhibited some degree of non-competitive inhibition.
In the presence of GdnHCl, galactose exhibited competitive inhibition at the lower [galactose] of 5 mM which changed to non-competitive as the [galactose] increased.
The implications of our findings are further discussed.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Nwamba, Charles O.& Chilaka, Ferdinand C.. 2012. Kinetic Analysis of Guanidine Hydrochloride Inactivation of β-Galactosidase in the Presence of Galactose. Enzyme Research،Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-451790
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Nwamba, Charles O.& Chilaka, Ferdinand C.. Kinetic Analysis of Guanidine Hydrochloride Inactivation of β-Galactosidase in the Presence of Galactose. Enzyme Research No. 2012 (2012), pp.1-13.
https://search.emarefa.net/detail/BIM-451790
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Nwamba, Charles O.& Chilaka, Ferdinand C.. Kinetic Analysis of Guanidine Hydrochloride Inactivation of β-Galactosidase in the Presence of Galactose. Enzyme Research. 2012. Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-451790
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-451790
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر