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Bioactivity Determination of Native and Variant Forms of Therapeutic Interferons
المؤلفون المشاركون
Diress, Abebaw
Li, Xuguang
Wang, Junzhi
Larocque, Louise
Bliu, Alex
Xu, Ranran
Lin, Rongtuan
Girard, Michel
He, Runtao
المصدر
Journal of Biomedicine and Biotechnology
العدد
المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2011-03-03
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
The traditional antiviral assays for the determination of interferon potency are reported to have considerable variability between and within assays.
Although several reporter gene assays based on interferon-inducible promoter activities have been reported, data from comprehensive validation studies are lacking and few studies have been conducted to analyze the variant forms of interferons, which could have undesirable clinical implications.
Here, a reporter gene assay employing a HEK293 cell line stably transfected with luciferase gene under the control of interferon-stimulated response element promoter was developed and validated.
The assay was found to be more sensitive, with a larger detection range than the antiviral assay.
Several cytokines tested did not interfere with the test, suggesting the assay possesses a certain degree of selectivity.
Moreover, the robustness of the assay was demonstrated by minimal variations in the results generated by different analysts and cell passage number (up to 52 passages).
Finally, the method was employed to analyze several interferon variants (interferon-α 2a) and we found that the aggregated form has completely lost its potency; while a modest loss of bioactivity in oxidized interferon was observed (approx.
23%), the deamidated form essentially retained its activity.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Larocque, Louise& Bliu, Alex& Xu, Ranran& Diress, Abebaw& Wang, Junzhi& Lin, Rongtuan…[et al.]. 2011. Bioactivity Determination of Native and Variant Forms of Therapeutic Interferons. Journal of Biomedicine and Biotechnology،Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-451860
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Larocque, Louise…[et al.]. Bioactivity Determination of Native and Variant Forms of Therapeutic Interferons. Journal of Biomedicine and Biotechnology No. 2011 (2011), pp.1-11.
https://search.emarefa.net/detail/BIM-451860
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Larocque, Louise& Bliu, Alex& Xu, Ranran& Diress, Abebaw& Wang, Junzhi& Lin, Rongtuan…[et al.]. Bioactivity Determination of Native and Variant Forms of Therapeutic Interferons. Journal of Biomedicine and Biotechnology. 2011. Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-451860
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-451860
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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