Proteome Changes in the Plasma of Myelodysplastic Syndrome Patients with Refractory Anemia with Excess Blasts Subtype 2
المؤلفون المشاركون
Riedelova-Reicheltova, Zuzana
Dyr, Jan E.
Pecankova, Klara
Suttnar, Jiri
Majek, Pavel
Cermak, Jaroslav
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-05-25
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
The goal of this study was to explore the plasma proteome of myelodysplastic syndrome (MDS) patients with refractory anemia with excess blasts subtype 2 (RAEB-2) in comparison to healthy controls.
20 plasma samples were separated with 2D electrophoresis and statistically processed with Progenesis SameSpots software.
47 significantly differing (P<0.05) spots were observed, and 27 different proteins were identified by nano-LC-MS/MS.
Mass spectrometry-based relative label-free quantification showed a 2-fold increase of the leucine-rich alpha-2-glycoprotein (LRAG) peptide levels in the RAEB-2 group.
Changes in the fragments of the inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4) protein were observed.
Western blot analysis showed no differences in albumin and ITIH4 levels, while increased expression was observed for LRAG in the RAEB-2 group.
Quantification using ELISA showed decreased plasma level of alpha-2-HS glycoprotein in the RAEB-2 group.
In conclusion, this is the first time that alpha-2-HS glycoprotein and LRAG were proposed as new biomarkers of RAEB-2 and advanced MDS, respectively.
Alpha-2-HS glycoprotein, a protein involved in the bone marrow development and previously proposed as a MDS biomarker candidate, was significantly decreased in RAEB-2.
Increased expression and changes in modification(s) were observed for LRAG, a protein involved in granulocytic and neutrophil differentiation, and angiogenesis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Majek, Pavel& Riedelova-Reicheltova, Zuzana& Suttnar, Jiri& Pecankova, Klara& Cermak, Jaroslav& Dyr, Jan E.. 2014. Proteome Changes in the Plasma of Myelodysplastic Syndrome Patients with Refractory Anemia with Excess Blasts Subtype 2. Disease Markers،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-452168
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Majek, Pavel…[et al.]. Proteome Changes in the Plasma of Myelodysplastic Syndrome Patients with Refractory Anemia with Excess Blasts Subtype 2. Disease Markers No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-452168
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Majek, Pavel& Riedelova-Reicheltova, Zuzana& Suttnar, Jiri& Pecankova, Klara& Cermak, Jaroslav& Dyr, Jan E.. Proteome Changes in the Plasma of Myelodysplastic Syndrome Patients with Refractory Anemia with Excess Blasts Subtype 2. Disease Markers. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-452168
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-452168
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر