Validation of Reference Genes for Normalization Gene Expression in Reverse Transcription Quantitative PCR in Human Normal Thyroid and Goiter Tissue

المؤلفون المشاركون

Brum, llma Simoni
Weber, Raquel
Bessestil, Laura Walter
Brasil, Beatriz Maria de Azevedo Assis
Furlanetto, Tania Weber
Bertoni, Ana Paula Santin

المصدر

BioMed Research International

العدد

المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-5، 5ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2014-05-11

دولة النشر

مصر

عدد الصفحات

5

التخصصات الرئيسية

الطب البشري

الملخص EN

Reverse transcription quantitative polymerase chain reaction (RT-qPCR) has been recognized as the most accurate method for quantifying mRNA transcripts, but normalization of samples is a prerequisite for correct data interpretation.

So, this study aimed to evaluate the most stable reference gene for RT-qPCR in human normal thyroid and goiter tissues.

Beta-actin (ACTB); glyceraldehyde-3-phosphate dehydrogenase (GAPDH); succinate dehydrogenase, subunit A, flavoprotein (Fp) (SDHA); hypoxanthine phosphoribosyltransferase I (HPRTI); tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide (YWHAZ); and beta-2-microglobulin (B2M) were evaluated in 14 thyroid tissue samples (7 normal and 7 goiter tissues) by RT-qPCR.

The mean Cq and the maximum fold change (MFC) and NormFinder software were used to assess the stability of the genes.

As a result, ACTB gene was more stable than GAPDH, SDHA, HPRTI, YWHAZ, and B2M.

In conclusion, ACTB could be used to normalize RT-qPCR data in normal thyroid and goiter tissues.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Weber, Raquel& Bertoni, Ana Paula Santin& Bessestil, Laura Walter& Brasil, Beatriz Maria de Azevedo Assis& Brum, llma Simoni& Furlanetto, Tania Weber. 2014. Validation of Reference Genes for Normalization Gene Expression in Reverse Transcription Quantitative PCR in Human Normal Thyroid and Goiter Tissue. BioMed Research International،Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-453902

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Weber, Raquel…[et al.]. Validation of Reference Genes for Normalization Gene Expression in Reverse Transcription Quantitative PCR in Human Normal Thyroid and Goiter Tissue. BioMed Research International No. 2014 (2014), pp.1-5.
https://search.emarefa.net/detail/BIM-453902

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Weber, Raquel& Bertoni, Ana Paula Santin& Bessestil, Laura Walter& Brasil, Beatriz Maria de Azevedo Assis& Brum, llma Simoni& Furlanetto, Tania Weber. Validation of Reference Genes for Normalization Gene Expression in Reverse Transcription Quantitative PCR in Human Normal Thyroid and Goiter Tissue. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-453902

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-453902