Characterization of Human Vaginal Mucosa Cells for Autologous In Vitro Cultured Vaginal Tissue Transplantation in Patients with MRKH Syndrome
المؤلفون المشاركون
Nodale, Cristina
Ceccarelli, Simona
Monti, Marco
Angeloni, Antonio
Vescarelli, Enrica
Maffucci, Diana
D’Amici, Sirio
Romano, Ferdinando
Benedetti Panici, Pierluigi
Marchese, Cinzia
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-08-05
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Mayer-Rokitansky-Küster-Hauser (MRKH) is a rare syndrome characterized by congenital aplasia of the uterus and vagina.
The most common procedure used for surgical reconstruction of the neovagina is the McIndoe vaginoplasty, which consists in creation of a vaginal canal covered with a full-thickness skin graft.
Here we characterized the autologous in vitro cultured vaginal tissue proposed as alternative material in our developed modified McIndoe vaginoplasty in order to underlie its importance in autologous total vaginal replacement.
To this aim human vaginal mucosa cells (HVMs) were isolated from vaginal mucosa of patients affected by MRKH syndrome and characterized with respect to growth kinetics, morphology, PAS staining, and expression of specific epithelial markers by immunofluorescence, Western blot, and qRT-PCR analyses.
The presence of specific epithelial markers along with the morphology and the presence of mucified cells demonstrated the epithelial nature of HMVs, important for an efficient epithelialization of the neovagina walls and for creating a functional vaginal cavity.
Moreover, these cells presented characteristics of effective proliferation as demonstrated by growth kinetics assay.
Therefore, the autologous in vitro cultured vaginal tissue might represent a highly promising and valid material for McIndoe vaginoplasty.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Nodale, Cristina& Vescarelli, Enrica& D’Amici, Sirio& Maffucci, Diana& Ceccarelli, Simona& Monti, Marco…[et al.]. 2014. Characterization of Human Vaginal Mucosa Cells for Autologous In Vitro Cultured Vaginal Tissue Transplantation in Patients with MRKH Syndrome. BioMed Research International،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-453988
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Nodale, Cristina…[et al.]. Characterization of Human Vaginal Mucosa Cells for Autologous In Vitro Cultured Vaginal Tissue Transplantation in Patients with MRKH Syndrome. BioMed Research International No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-453988
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Nodale, Cristina& Vescarelli, Enrica& D’Amici, Sirio& Maffucci, Diana& Ceccarelli, Simona& Monti, Marco…[et al.]. Characterization of Human Vaginal Mucosa Cells for Autologous In Vitro Cultured Vaginal Tissue Transplantation in Patients with MRKH Syndrome. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-453988
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-453988
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر