Zeaxanthin Induces Apoptosis in Human Uveal Melanoma Cells through Bcl-2 Family Proteins and Intrinsic Apoptosis Pathway

المؤلفون المشاركون

Hu, Dan-Ning
Song, E.
McCormick, Steven A.
Bi, Ming-Chao
Rosen, Richard
Zha, Ren-Yuan

المصدر

Evidence-Based Complementary and Alternative Medicine

العدد

المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-12، 12ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2013-10-10

دولة النشر

مصر

عدد الصفحات

12

التخصصات الرئيسية

الطب البشري

الملخص EN

The cytotoxic effects of zeaxanthin on two human uveal melanoma cell lines (SP6.5 and C918) and related signaling pathways were studied and compared to effects on normal ocular cells (uveal melanocytes, retinal pigment epithelial cells, and scleral fibroblasts).

MTT assay revealed that zeaxanthin reduced the cell viability of melanoma cells in a dose-dependent manner (10, 30, and 100 μM), with IC50 at 40.8 and 28.7 μM in SP6.5 and C918 cell lines, respectively.

Zeaxanthin did not affect the viability of normal ocular cells even at the highest levels tested (300 μM), suggesting that zeaxanthin has a selectively cytotoxic effect on melanoma cells.

Zeaxanthin induced apoptosis in melanoma cells as indicated by annexin V and ethidium III flow cytometry.

Western blot analysis demonstrated that zeaxanthin decreased the expression of antiapoptotic proteins (Bcl-2 and Bcl-xL) and increased the expression of proapoptotic proteins (Bak and Bax) in zeaxanthin-treated melanoma cells.

Zeaxanthin increased mitochondrial permeability as determined by JC-1 fluorescein study.

Zeaxanthin also increased the level of cytosol cytochrome c and caspase-9 and -3 activities, but not caspase-8, as measured by ELISA assay or colorimetric assay.

All of these findings indicate that the intrinsic (mitochondrial) pathway is involved in zeaxanthin-induced apoptosis in uveal melanoma cells.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Bi, Ming-Chao& Rosen, Richard& Zha, Ren-Yuan& McCormick, Steven A.& Song, E.& Hu, Dan-Ning. 2013. Zeaxanthin Induces Apoptosis in Human Uveal Melanoma Cells through Bcl-2 Family Proteins and Intrinsic Apoptosis Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-454310

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Bi, Ming-Chao…[et al.]. Zeaxanthin Induces Apoptosis in Human Uveal Melanoma Cells through Bcl-2 Family Proteins and Intrinsic Apoptosis Pathway. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-454310

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Bi, Ming-Chao& Rosen, Richard& Zha, Ren-Yuan& McCormick, Steven A.& Song, E.& Hu, Dan-Ning. Zeaxanthin Induces Apoptosis in Human Uveal Melanoma Cells through Bcl-2 Family Proteins and Intrinsic Apoptosis Pathway. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-454310

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-454310