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Short-Term Exposure to Tobacco Toxins Alters Expression of Multiple Proliferation Gene Markers in Primary Human Bronchial Epithelial Cell Cultures
المؤلفون المشاركون
Chaudhry, Imran S.
Kass, Lawrence
Machekano, Rhoderick N.
El-Meanawy, Ashraf
Khiyami, Amer
Tomashefski, Joseph F.
المصدر
العدد
المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2011-04-05
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
The biological effects of only a finite number of tobacco toxins have been studied.
Here, we describe exposure of cultures of human bronchial epithelial cells to low concentrations of tobacco carcinogens: nickel sulphate, benzo(b)fluoranthene, N-nitrosodiethylamine, and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK).
After a 24-hour exposure, EGFR was expressed in cell membrane and cytoplasm, BCL-2 was expressed only in the irregular nuclei of large atypical cells, MKI67 was expressed in nuclei with no staining in larger cells, cytoplasmic BIRC5 with stronger nuclear staining was seen in large atypical cells, and nuclear TP53 was strongly expressed in all cells.
After only a 24-hour exposure, cells exhibited atypical nuclear and cytoplasmic features.
After a 48-hour exposure, EGFR staining was localized to the nucleus, BCL-2 was slightly decreased in intensity, BIRC5 was localized to the cytoplasm, and TP53 staining was increased in small and large cells.
BCL2L1 was expressed in both the cytoplasm and nuclei of cells at 24- and 48-hour exposures.
We illustrate that short-termexposure of a bronchial epithelial cell line to smoking-equivalent concentrations of tobacco carcinogens alters the expression of key proliferation regulatory genes, EGFR, BCL-2, BCL2L1, BIRC5, TP53, and MKI67, similar to that reported in biopsy specimens of pulmonary epithelium described to be preneoplastic lesions.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chaudhry, Imran S.& El-Meanawy, Ashraf& Khiyami, Amer& Tomashefski, Joseph F.& Machekano, Rhoderick N.& Kass, Lawrence. 2011. Short-Term Exposure to Tobacco Toxins Alters Expression of Multiple Proliferation Gene Markers in Primary Human Bronchial Epithelial Cell Cultures. Journal of Oncology،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-454663
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chaudhry, Imran S.…[et al.]. Short-Term Exposure to Tobacco Toxins Alters Expression of Multiple Proliferation Gene Markers in Primary Human Bronchial Epithelial Cell Cultures. Journal of Oncology No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-454663
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chaudhry, Imran S.& El-Meanawy, Ashraf& Khiyami, Amer& Tomashefski, Joseph F.& Machekano, Rhoderick N.& Kass, Lawrence. Short-Term Exposure to Tobacco Toxins Alters Expression of Multiple Proliferation Gene Markers in Primary Human Bronchial Epithelial Cell Cultures. Journal of Oncology. 2011. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-454663
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-454663
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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