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Strain-Dependent Induction of Human Enterocyte Apoptosis by Blastocystis Disrupts Epithelial Barrier and ZO-1 Organization in a Caspase 3- and 9-Dependent Manner
المؤلفون المشاركون
Teo, Joshua D. W.
Mirza, Haris
Wu, Zhaona
Tan, Kevin S. W.
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-04-14
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Blastocystis is an emerging protistan parasite colonizing the human intestine.
It is frequently reported to cause general intestinal symptoms of vomiting, diarrhea, and abdominal pain.
We recently demonstrated that Blastocystis rearranged cytoskeletal proteins and induced intestinal epithelial barrier compromise.
The effect of Blastocystis on enterocyte apoptosis is unknown, and a possible link between microbially induced enterocyte apoptosis and increased epithelial permeability has yet to be determined.
The aim of this study is to assess if Blastocystis induces human enterocyte apoptosis and whether this effect influences human intestinal epithelial barrier function.
Monolayers of polarized human colonic epithelial cell-line Caco-2 were incubated with Blastocystis subtype 7 and subtype 4.
Assays for both early and late markers of apoptosis, phosphatidylserine externalization, and nuclear fragmentation, respectively, showed that Blastocystis ST-7, but not ST-4, significantly increased apoptosis in enterocytes, suggesting that Blastocystis exhibits host specificity and strain-to-strain variation in pathogenicity.
ST-7 also activated Caco-2 caspases 3 and 9 but not 8.
ST-7 induced changes in epithelial resistance, permeability, and tight junction (ZO-1) localization.
Pretreatment of Caco-2 monolayers with a pan-caspase inhibitor z-VAD-fmk significantly inhibited these changes.
This suggests a role for enterocyte apoptosis in Blastocystis-mediated epithelial barrier compromise in the human intestine.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wu, Zhaona& Mirza, Haris& Teo, Joshua D. W.& Tan, Kevin S. W.. 2014. Strain-Dependent Induction of Human Enterocyte Apoptosis by Blastocystis Disrupts Epithelial Barrier and ZO-1 Organization in a Caspase 3- and 9-Dependent Manner. BioMed Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-454712
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wu, Zhaona…[et al.]. Strain-Dependent Induction of Human Enterocyte Apoptosis by Blastocystis Disrupts Epithelial Barrier and ZO-1 Organization in a Caspase 3- and 9-Dependent Manner. BioMed Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-454712
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wu, Zhaona& Mirza, Haris& Teo, Joshua D. W.& Tan, Kevin S. W.. Strain-Dependent Induction of Human Enterocyte Apoptosis by Blastocystis Disrupts Epithelial Barrier and ZO-1 Organization in a Caspase 3- and 9-Dependent Manner. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-454712
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-454712
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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