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Cotransduction with MGMT and Ubiquitous or Erythroid-Specific GFP Lentiviruses Allows Enrichment of Dual-Positive Hematopoietic Progenitor Cells In Vivo
المؤلفون المشاركون
Reese, Jane S.
Gerson, Stanton L.
Ismail, Mourad
Lingas, Karen T.
Roth, Justin C.
Ferrari, Giuliana
المصدر
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-07-19
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
The P140K point mutant of MGMT allows robust hematopoietic stem cell (HSC) enrichment in vivo.
Thus, dual-gene vectors that couple MGMT and therapeutic gene expression have allowed enrichment of gene-corrected HSCs in animal models.
However, expression levels from dual-gene vectors are often reduced for one or both genes.
Further, it may be desirable to express selection and therapeutic genes at distinct stages of cell differentiation.
In this regard, we evaluated whether hematopoietic cells could be efficiently cotransduced using low MOIs of two separate single-gene lentiviruses, including MGMT for dual-positive cell enrichment.
Cotransduction efficiencies were evaluated using a range of MGMT : GFP virus ratios, MOIs, and selection stringencies in vitro.
Cotransduction was optimal when equal proportions of each virus were used, but low MGMT : GFP virus ratios resulted in the highest proportion of dual-positive cells after selection.
This strategy was then evaluated in murine models for in vivo selection of HSCs cotransduced with a ubiquitous MGMT expression vector and an erythroid-specific GFP vector.
Although the MGMT and GFP expression percentages were variable among engrafted recipients, drug selection enriched MGMT-positive leukocyte and GFP-positive erythroid cell populations.
These data demonstrate cotransduction as a mean to rapidly enrich and evaluate therapeutic lentivectors in vivo.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Roth, Justin C.& Ismail, Mourad& Reese, Jane S.& Lingas, Karen T.& Ferrari, Giuliana& Gerson, Stanton L.. 2012. Cotransduction with MGMT and Ubiquitous or Erythroid-Specific GFP Lentiviruses Allows Enrichment of Dual-Positive Hematopoietic Progenitor Cells In Vivo. ISRN Hematology،Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-454943
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Roth, Justin C.…[et al.]. Cotransduction with MGMT and Ubiquitous or Erythroid-Specific GFP Lentiviruses Allows Enrichment of Dual-Positive Hematopoietic Progenitor Cells In Vivo. ISRN Hematology No. 2012 (2012), pp.1-12.
https://search.emarefa.net/detail/BIM-454943
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Roth, Justin C.& Ismail, Mourad& Reese, Jane S.& Lingas, Karen T.& Ferrari, Giuliana& Gerson, Stanton L.. Cotransduction with MGMT and Ubiquitous or Erythroid-Specific GFP Lentiviruses Allows Enrichment of Dual-Positive Hematopoietic Progenitor Cells In Vivo. ISRN Hematology. 2012. Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-454943
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-454943
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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