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Production of Fungal Amylases Using Cheap, Readily Available Agriresidues, for Potential Application in Textile Industry
المؤلفون المشاركون
Bali, Vrinda
Singh, Sanamdeep
Mangla, Jyoti
Singh, Shalini
Sharma, Lovleen
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-01-09
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
The study aimed at isolation and screening of fungal amylase producer, optimization of solid state fermentation conditions for maximum amylase production by the best amylase producer, and characterization of the crude amylases, so produced.
Aspergillus fumigatus NTCC1222 showed the highest amylase activity (164.1 U/mL) in secondary screening under SSF conditions and was selected for further studies.
The test strain showed maximum amylase production (341.7 U/mL) and supernatant protein concentration (9.7 mg/mL) for incubation period (6 days), temperature (35°C), initial pH (6.0), nutrient salt solution as moistening agent, and beef extract as nitrogen source.
Pomegranate peel produced maximum amylase activity, but wheat bran (only slightly lesser amylase activity as compared to that of pomegranate peel) was chosen for further studies, keeping in mind the seasonal availability of pomegranate peel.
TLC confirmed the amylase produced to be α-type and 60 kDa was the molecular weight of the partially purified amylase.
The enzyme showed maximum enzyme activity at pH 6.0, temperature of 55°C, and incubation time of 60 minutes.
UV (616.0 U/mL) and chemical (814.2 U/mL) mutation enhanced amylase activity as compared to wild test strain.
The study indicates that Aspergillus fumigatus NTCC1222 can be an important source of amylase and the crude enzyme, hence obtained, can be cost effectively applied in multiple sections of textile wet processing.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Singh, Shalini& Singh, Sanamdeep& Bali, Vrinda& Sharma, Lovleen& Mangla, Jyoti. 2014. Production of Fungal Amylases Using Cheap, Readily Available Agriresidues, for Potential Application in Textile Industry. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-455234
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Singh, Shalini…[et al.]. Production of Fungal Amylases Using Cheap, Readily Available Agriresidues, for Potential Application in Textile Industry. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-455234
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Singh, Shalini& Singh, Sanamdeep& Bali, Vrinda& Sharma, Lovleen& Mangla, Jyoti. Production of Fungal Amylases Using Cheap, Readily Available Agriresidues, for Potential Application in Textile Industry. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-455234
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-455234
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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