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Molecular Characterization of the 16S rRNA Gene of Helicobacter fennelliae Isolated from Stools and Blood Cultures from Paediatric Patients in South Africa
المؤلفون المشاركون
Smuts, Heidi E. M.
Lastovica, Albert Joseph
المصدر
العدد
المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2010-11-29
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Forty strains of H.
fennelliae collected from paediatric blood and stool samples over an 18 year period at a children's hospital in Cape Town, South Africa, were amplified by PCR of the 16S rRNA.
Two distinct genotypes of H.
fennelliae were identified based on the phylogenetic analysis.
This was confirmed by sequencing a portion of the beta subunit of the RNA polymerase (rpoB) gene.
All isolates from South Africa clustered with a proposed novel Helicobacter strain (accession number AF237612) isolated in Australia, while three H.
fennelliae type strains from the northern hemisphere, NCTC 11612, LMG 7546 and CCUG 18820, formed a separate branch.
A large (355bp) highly conserved intervening sequence (IVS) in the 16S rRNA was found in all isolates.
Predicted secondary structures of the IVS from the 16S rRNA and 23S rRNA were characterised by a primary stem structure formed by base pairing of the 3′ and 5′ ends and internal loops and stems.
This phylogenetic analysis is the largest undertaken of H.
fennelliae.
The South African H.
fennelliae isolates are closely related to an Australian isolate previously reported to be a possible novel species of Helicobacter.
This study suggests that the latter is strain of H.
fennelliae.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Smuts, Heidi E. M.& Lastovica, Albert Joseph. 2010. Molecular Characterization of the 16S rRNA Gene of Helicobacter fennelliae Isolated from Stools and Blood Cultures from Paediatric Patients in South Africa. Journal of Pathogens،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-455392
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Smuts, Heidi E. M.& Lastovica, Albert Joseph. Molecular Characterization of the 16S rRNA Gene of Helicobacter fennelliae Isolated from Stools and Blood Cultures from Paediatric Patients in South Africa. Journal of Pathogens No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-455392
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Smuts, Heidi E. M.& Lastovica, Albert Joseph. Molecular Characterization of the 16S rRNA Gene of Helicobacter fennelliae Isolated from Stools and Blood Cultures from Paediatric Patients in South Africa. Journal of Pathogens. 2010. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-455392
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-455392
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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