Effects of Essential Oils and Polyunsaturated Fatty Acids on Canine Skin Equivalents : Skin Lipid Assessment and Morphological Evaluation
المؤلفون المشاركون
Rodes, D.
Cerrato, S.
Fondevila, D.
Puigdemont, A.
Ramió-Lluch, L.
Brazis, P.
المصدر
Journal of Veterinary Medicine
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-11-06
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
A canine skin equivalent model has been validated for the assessment of a topical formulation effects.
Skin equivalents were developed from freshly isolated cutaneous canine fibroblasts and keratinocytes, after enzymatic digestion of skin samples (n=8) from different breeds.
Fibroblasts were embedded into a collagen type I matrix, and keratinocytes were seeded onto its surface at air-liquid interface.
Skin equivalents were supplemented with essential oils and polyunsaturated fatty acid formulation or with vehicle.
Skin equivalents were histopathologically and ultrastructurally studied, and the three main lipid groups (free fatty acids, cholesterol, and ceramides) were analyzed.
Results showed that the culture method developed resulted in significant improvements in cell retrieval and confluence.
Treated samples presented a thicker epidermis with increased number of viable cell layers, a denser and compact stratum corneum, and a more continuous basal membrane.
Regarding lipid profile, treated skin equivalents showed a significant increase in ceramide content (51.7±1.3) when compared to untreated (41.6 ± 1.4) samples.
Ultrastructural study evidenced a compact and well-organized stratum corneum in both treated and control skin equivalents.
In conclusion, cell viability and ceramides increase, after lipid supplementation, are especially relevant for the treatment of skin barrier disruptions occurring in canine atopic dermatitis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Cerrato, S.& Ramió-Lluch, L.& Fondevila, D.& Rodes, D.& Brazis, P.& Puigdemont, A.. 2013. Effects of Essential Oils and Polyunsaturated Fatty Acids on Canine Skin Equivalents : Skin Lipid Assessment and Morphological Evaluation. Journal of Veterinary Medicine،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-455799
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Cerrato, S.…[et al.]. Effects of Essential Oils and Polyunsaturated Fatty Acids on Canine Skin Equivalents : Skin Lipid Assessment and Morphological Evaluation. Journal of Veterinary Medicine No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-455799
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Cerrato, S.& Ramió-Lluch, L.& Fondevila, D.& Rodes, D.& Brazis, P.& Puigdemont, A.. Effects of Essential Oils and Polyunsaturated Fatty Acids on Canine Skin Equivalents : Skin Lipid Assessment and Morphological Evaluation. Journal of Veterinary Medicine. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-455799
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-455799
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر