Exploration of the Lysis Mechanisms of Leukaemic Blasts by Chimaeric T-Cells
المؤلفون المشاركون
Chaperot, Laurence
Biagi, Ettore
Plumas, Joël
Jacob, Marie Christine
Gallot, Géraldine
Agostoni, Valentina
Pizzitola, Irene
Vié, Henri
Laurin, David
Aspord, Caroline
Marin, Virna
المصدر
Journal of Biomedicine and Biotechnology
العدد
المجلد 2010، العدد 2010 (31 ديسمبر/كانون الأول 2010)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2010-06-13
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Adoptive transfer of specific cytotoxic T lymphocytes (CTL) and Cytokine Induced Killer Cells (CIK) following genetic engineering of T-cell receptor zeta hold promising perspective in immunotherapy.
In the present work we focused on the mechanisms of anti-tumor action of effectors transduced with an anti-CD19 chimaeric receptor in the context of B-lineage acute lymphoblastic leukemia (B-ALL).
Primary B-ALL blasts were efficiently killed by both z-CD19 CTL and z-CD19 CIK effectors.
The use of death receptor mediated apoptosis of target cells was excluded since agonists molecules of Fas and TRAIL-receptors failed to induce cell death.
Perforin/granzyme pathway was found to be the mechanism of chimaeric effectors mediated killing.
Indeed, cytolytic effector molecules perforin as well as granzymes were highly expressed by CTL and CIK.
CD19 specific stimulation of transduced effectors was associated with degranulation as attested by CD107 membrane expression and high IFN-γ and TNF-α release.
Moreover inhibitors of the perforin-based cytotoxic pathway, Ca2+-chelating agent EGTA and Concanamycin A, almost completely abrogated B-ALL blast killing.
In conclusion we show that the cytolysis response of z-CD19 chimaeric effectors is predominantly mediated via perforin/granzyme pathway and is independent of death receptors signaling in primary B-ALL.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Laurin, David& Marin, Virna& Biagi, Ettore& Pizzitola, Irene& Agostoni, Valentina& Gallot, Géraldine…[et al.]. 2010. Exploration of the Lysis Mechanisms of Leukaemic Blasts by Chimaeric T-Cells. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-455984
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Laurin, David…[et al.]. Exploration of the Lysis Mechanisms of Leukaemic Blasts by Chimaeric T-Cells. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-9.
https://search.emarefa.net/detail/BIM-455984
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Laurin, David& Marin, Virna& Biagi, Ettore& Pizzitola, Irene& Agostoni, Valentina& Gallot, Géraldine…[et al.]. Exploration of the Lysis Mechanisms of Leukaemic Blasts by Chimaeric T-Cells. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-455984
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-455984
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر