The Effects of Brazilian Green Propolis against Excessive Light-Induced Cell Damage in Retina and Fibroblast Cells

المؤلفون المشاركون

Ichihara, Kenji
Tsuruma, Kazuhiro
Hara, Hideaki
Kakino, Mamoru
Shimazawa, Masamitsu
Murase, Hiromi

المصدر

Evidence-Based Complementary and Alternative Medicine

العدد

المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-11، 11ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2013-12-12

دولة النشر

مصر

عدد الصفحات

11

التخصصات الرئيسية

الطب البشري

الملخص EN

Background.

We investigated the effects of Brazilian green propolis and its constituents against white light- or UVA-induced cell damage in mouse retinal cone-cell line 661W or human skin-derived fibroblast cells (NB1-RGB).

Methods.

Cell damage was induced by 3,000lx white light for 24 h or 4/10 J/cm2 UVA exposure.

Cell viability was assessed by Hoechst33342 and propidium iodide staining or by tetrazolium salt (WST-8) cell viability assay.

The radical scavenging activity of propolis induced by UVA irradiation in NB1-RGB cells was measured using a reactive-oxygen-species- (ROS-) sensitive probe CM-H2DCFDA.

Moreover, the effects of propolis on the UVA-induced activation of p38 and extracellular signal-regulated kinase (ERK) were examined by immunoblotting.

Results.

Treatment with propolis and two dicaffeoylquinic acids significantly inhibited the decrease in cell viability induced by white light in 661W.

Propolis and its constituents inhibited the decrease in cell viability induced by UVA in NB1-RGB.

Moreover, propolis suppressed the intracellular ROS production by UVA irradiation.

Propolis also inhibited the levels of phosphorylated-p38 and ERK by UVA irradiation.

Conclusion.

Brazilian green propolis may become a major therapeutic candidate for the treatment of AMD and skin damage induced by UV irradiation.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Murase, Hiromi& Shimazawa, Masamitsu& Kakino, Mamoru& Ichihara, Kenji& Tsuruma, Kazuhiro& Hara, Hideaki. 2013. The Effects of Brazilian Green Propolis against Excessive Light-Induced Cell Damage in Retina and Fibroblast Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-456320

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Murase, Hiromi…[et al.]. The Effects of Brazilian Green Propolis against Excessive Light-Induced Cell Damage in Retina and Fibroblast Cells. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-456320

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Murase, Hiromi& Shimazawa, Masamitsu& Kakino, Mamoru& Ichihara, Kenji& Tsuruma, Kazuhiro& Hara, Hideaki. The Effects of Brazilian Green Propolis against Excessive Light-Induced Cell Damage in Retina and Fibroblast Cells. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-456320

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-456320