Relative Quantification of Several Plasma Proteins during Liver Transplantation Surgery
المؤلفون المشاركون
Renkonen, Risto
Ilmakunnas, Minna
Isoniemi, Helena
Joenväärä, Sakari
Parviainen, Ville
Tukiainen, Eija
المصدر
Journal of Biomedicine and Biotechnology
العدد
المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2011-12-10
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Plasma proteome is widely used in studying changes occurring in human body during disease or other disturbances.
Immunological methods are commonly used in such studies.
In recent years, mass spectrometry has gained popularity in high-throughput analysis of plasma proteins.
In this study, we tested whether mass spectrometry and iTRAQ-based protein quantification might be used in proteomic analysis of human plasma during liver transplantation surgery to characterize changes in protein abundances occurring during early graft reperfusion.
We sampled blood from systemic circulation as well as blood entering and exiting the liver.
After immunodepletion of six high-abundant plasma proteins, trypsin digestion, iTRAQ labeling, and cation-exchange fractionation, the peptides were analyzed by reverse phase nano-LC-MS/MS.
In total, 72 proteins were identified of which 31 could be quantified in all patient specimens collected.
Of these 31 proteins, ten, mostly medium-to-high abundance plasma proteins with a concentration range of 50–2000 mg/L, displayed relative abundance change of more than 10%.
The changes in protein abundance observed in this study allow further research on the role of several proteins in ischemia-reperfusion injury during liver transplantation and possibly in other surgery.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Parviainen, Ville& Joenväärä, Sakari& Tukiainen, Eija& Ilmakunnas, Minna& Isoniemi, Helena& Renkonen, Risto. 2011. Relative Quantification of Several Plasma Proteins during Liver Transplantation Surgery. Journal of Biomedicine and Biotechnology،Vol. 2011, no. 2011, pp.1-12.
https://search.emarefa.net/detail/BIM-457218
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Parviainen, Ville…[et al.]. Relative Quantification of Several Plasma Proteins during Liver Transplantation Surgery. Journal of Biomedicine and Biotechnology No. 2011 (2011), pp.1-12.
https://search.emarefa.net/detail/BIM-457218
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Parviainen, Ville& Joenväärä, Sakari& Tukiainen, Eija& Ilmakunnas, Minna& Isoniemi, Helena& Renkonen, Risto. Relative Quantification of Several Plasma Proteins during Liver Transplantation Surgery. Journal of Biomedicine and Biotechnology. 2011. Vol. 2011, no. 2011, pp.1-12.
https://search.emarefa.net/detail/BIM-457218
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-457218
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر