![](/images/graphics-bg.png)
Time Exposure Period of Bovine Oocytes to Sperm in Relation to Embryo Development Rate and Quality
المؤلفون المشاركون
Peralta, Oscar
Ratto, Marcelo
Berland, Marco
Frei, Mario
المصدر
العدد
المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-4، 4ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2011-02-24
دولة النشر
مصر
عدد الصفحات
4
التخصصات الرئيسية
الملخص EN
The objective of the study was to determine the effect of different bovine gamete coincubation times on fertilization and embryo development performance.
In vitro matured COCs were co-incubated with sperm at a concentration of 1.5 × 106 spermatozoa/ml in TALP medium for 3 hours (T 3, n=362), 6 hours (T 6, n=358), or 18 hours (T 18, n=350).
At the end of the coincubation period COCs from times 3 and 6 groups were post-incubated in a new well of fertilization medium without sperm for additional 15 and 12 h, respectively.
Cumulus Oocyte Complexes from the T 18 were co-incubated with the sperm suspension for 18 hours.
Presumptive zygotes were cultured for 9 days and embryo development was evaluated on days 2, 8, and 9.
Thirty blastocysts from each group were stained and total number of nuclei was recorded.
The mean (± SEM) percentages of zygotes to develop into ≥2 cell stage were 71.9 ± 5.0; 72.5 ± 5.3 and 81.2 ± 6.1 % for T 3, 6, and 18, respectively, on day 2 and they did not differ (P=.3) among groups.
The mean percentage of blastocysts developed on day 8 (25.6 ± 2.8; 24.2 ± 3.3; 28.4 ± 4.2 % for T 3, 6, and 18, resp.) did not differ (P=.4) among groups.
The total number of embryonic nuclei was greater (P<.05) for the blastocysts produced from the shortest co-incubation time (T 3).
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Berland, Marco& Frei, Mario& Peralta, Oscar& Ratto, Marcelo. 2011. Time Exposure Period of Bovine Oocytes to Sperm in Relation to Embryo Development Rate and Quality. ISRN Veterinary Science،Vol. 2011, no. 2011, pp.1-4.
https://search.emarefa.net/detail/BIM-457990
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Berland, Marco…[et al.]. Time Exposure Period of Bovine Oocytes to Sperm in Relation to Embryo Development Rate and Quality. ISRN Veterinary Science No. 2011 (2011), pp.1-4.
https://search.emarefa.net/detail/BIM-457990
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Berland, Marco& Frei, Mario& Peralta, Oscar& Ratto, Marcelo. Time Exposure Period of Bovine Oocytes to Sperm in Relation to Embryo Development Rate and Quality. ISRN Veterinary Science. 2011. Vol. 2011, no. 2011, pp.1-4.
https://search.emarefa.net/detail/BIM-457990
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-457990
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)