Attenuated Salmonella typhimurium SV4089 as a Potential Carrier of Oral DNA Vaccine in Chickens
المؤلفون المشاركون
Omar, Abdul Rahman
Zakaria, Zunita
Jazayeri, Seyed Davoud
Ideris, Aini
المصدر
Journal of Biomedicine and Biotechnology
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-06-04
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Attenuated Salmonella has been used as a carrier for DNA vaccine.
However, in vitro and in vivo studies on the bacteria following transfection of plasmid DNA were poorly studied.
In this paper, eukaryotic expression plasmids encoding avian influenza virus (AIV) subtype H5N1 genes, pcDNA3.1/HA, NA, and NP, were transfected into an attenuated Salmonella enteric typhimurium SV4089.
In vitro stability of the transfected plasmids into Salmonella were over 90% after 100 generations.
The attenuated Salmonella were able to invade MCF-7 (1.2%) and MCF-10A (0.5%) human breast cancer cells.
Newly hatched specific-pathogen-free (SPF) chicks were inoculated once by oral gavage with 109 colony-forming unit (CFU) of the attenuated Salmonella.
No abnormal clinical signs or deaths were recorded after inoculation.
Viable bacteria were detected 3 days after inoculation by plating from spleen, liver, and cecum.
Fluorescent in situ hybridization (FISH) and polymerase chain reaction (PCR) were carried out for confirmation.
Salmonella was not detected in blood cultures although serum antibody immune responses to Salmonella O antiserum group D1 factor 1, 9, and 12 antigens were observed in all the inoculated chickens after 7 days up to 35 days.
Our results showed that live attenuated S.
typhimurium SV4089 harboring pcDNA3.1/HA, NA, and NP may provide a unique alternative as a carrier for DNA oral vaccine in chickens.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Jazayeri, Seyed Davoud& Ideris, Aini& Zakaria, Zunita& Omar, Abdul Rahman. 2012. Attenuated Salmonella typhimurium SV4089 as a Potential Carrier of Oral DNA Vaccine in Chickens. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-458696
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Jazayeri, Seyed Davoud…[et al.]. Attenuated Salmonella typhimurium SV4089 as a Potential Carrier of Oral DNA Vaccine in Chickens. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-458696
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Jazayeri, Seyed Davoud& Ideris, Aini& Zakaria, Zunita& Omar, Abdul Rahman. Attenuated Salmonella typhimurium SV4089 as a Potential Carrier of Oral DNA Vaccine in Chickens. Journal of Biomedicine and Biotechnology. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-458696
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-458696
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر