Lipopolysaccharide Stimulates p62-Dependent Autophagy-Like Aggregate Clearance in Hepatocytes
المؤلفون المشاركون
Chen, Christine
Loughran, Patricia
Billiar, Timothy R.
Sun, Qian
Scott, Melanie J.
Deng, Meihong
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-02-10
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Impairment of autophagy has been associated with liver injury.
TLR4-stimulation by LPS upregulates autophagy in hepatocytes, although the signaling pathways involved remain elusive.
The objective of this study was to determine the signaling pathway leading to LPS-stimulated autophagy in hepatocytes.
Cell lysates from livers of wild type (WT; C57BL/6) mice given LPS (5 mg/kg-IP) and hepatocytes from WT, TLR4ko, and MyD88ko mice treated with LPS (100 ng/mL) up to 24 h were collected.
LC3II, p62/SQSTM1, Nrf2, and beclin1 levels were determined by immunoblot, immunofluorescence, and qPCR.
Autophagy-like activation was measured by GFP-LC3-puncta formation and LC3II-expression.
Beclin1, Nrf2, p62, MyD88, and TIRAP were knocked-down using siRNA.
LC3II-expression increased in both liver and hepatocytes after LPS and was dependent on TLR4.
Beclin1 expression did not increase after LPS in hepatocytes and beclin1-knockdown did not affect LC3II levels.
In hepatocytes given LPS, expression of p62 increased and p62 colocalized with LC3.
p62-knockdown prevented LC3II puncta formation.
LPS-induced LC3II/p62-puncta also required MyD88/TIRAP signaling and localization of both Nrf2 and NFκB transcription factors to the nucleus to upregulate p62-expression.
Therefore, TLR4-activation by LPS in hepatocytes induces a p62-mediated, not beclin1-mediated, autophagy-like clearance pathway that is hepatoprotective by clearing aggregate-prone or misfolded proteins from the cytosol and preserving energy homeostasis under stress.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chen, Christine& Deng, Meihong& Sun, Qian& Loughran, Patricia& Billiar, Timothy R.& Scott, Melanie J.. 2014. Lipopolysaccharide Stimulates p62-Dependent Autophagy-Like Aggregate Clearance in Hepatocytes. BioMed Research International،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-458820
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chen, Christine…[et al.]. Lipopolysaccharide Stimulates p62-Dependent Autophagy-Like Aggregate Clearance in Hepatocytes. BioMed Research International No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-458820
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chen, Christine& Deng, Meihong& Sun, Qian& Loughran, Patricia& Billiar, Timothy R.& Scott, Melanie J.. Lipopolysaccharide Stimulates p62-Dependent Autophagy-Like Aggregate Clearance in Hepatocytes. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-458820
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-458820
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر