Molecular Identification of Fungal Communities in a Soil Cultivated with Vegetables and Soil Suppressiveness to Rhizoctonia solani
المؤلفون المشاركون
Carareto-Alves, Lucia Maria
Val-Moraes, Silvana Pompéia
Nascimbem Pedrinho, Eliamar Aparecida
Lemos, Eliana Gertrudes de Macedo
المصدر
Applied and Environmental Soil Science
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-08-01
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Fungi constitute an important part of the soil ecosystem, playing key roles in decomposition, cycling processes, and biotic interactions.
Molecular methods have been used to assess fungal communities giving a more realistic view of their diversity.
For this purpose, total DNA was extracted from bulk soils cultivated with tomato (STC), vegetables (SHC), and native forest (SMS) from three sites of the Taquara Branca river basin in Sumaré County, São Paulo State, Brazil.
This metagenomic DNA was used as a template to amplify fungal 18S rDNA sequences, and libraries were constructed in Escherichia coli by cloning PCR products.
The plasmid inserts were sequenced and compared to known rDNA sequences in the GenBank database.
Of the sequenced clones, 22 were obtained from the SMS sample, 18 from the SHC sample, and 6 from the STC sample.
Although most of the clone sequences did not match the sequences present in the database, individual amplified sequences matched with Glomeromycota (SMS), Fungi incertae sedis (SMS), and Neocallimastigomycota (SHC).
Most of the sequences from the amplified taxa represent uncultured fungi.
The molecular analysis of variance (AMOVA) indicated that fluctuations observed of haplotypes in the composition may be related to herbicide application.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Val-Moraes, Silvana Pompéia& Nascimbem Pedrinho, Eliamar Aparecida& Lemos, Eliana Gertrudes de Macedo& Carareto-Alves, Lucia Maria. 2013. Molecular Identification of Fungal Communities in a Soil Cultivated with Vegetables and Soil Suppressiveness to Rhizoctonia solani. Applied and Environmental Soil Science،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-458958
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Val-Moraes, Silvana Pompéia…[et al.]. Molecular Identification of Fungal Communities in a Soil Cultivated with Vegetables and Soil Suppressiveness to Rhizoctonia solani. Applied and Environmental Soil Science No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-458958
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Val-Moraes, Silvana Pompéia& Nascimbem Pedrinho, Eliamar Aparecida& Lemos, Eliana Gertrudes de Macedo& Carareto-Alves, Lucia Maria. Molecular Identification of Fungal Communities in a Soil Cultivated with Vegetables and Soil Suppressiveness to Rhizoctonia solani. Applied and Environmental Soil Science. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-458958
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-458958
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر