Top-Down Characterization of the Post-Translationally Modified Intact Periplasmic Proteome from the Bacterium Novosphingobium aromaticivorans

المؤلفون المشاركون

Shukla, Anil K.
Moore, Ronald J.
Zhao, Rui
Cao, Li
Brown, Roslyn N.
Payne, Samuel H.
Lipton, Mary S.
Monroe, Matthew E.
Tolić, Nikola
Meng, Da
Wu, Si
Pasa-Tolic, Ljiljiana

المصدر

International Journal of Proteomics

العدد

المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-10، 10ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2013-03-10

دولة النشر

مصر

عدد الصفحات

10

التخصصات الرئيسية

العلوم الطبيعية والحياتية (متداخلة التخصصات)
الأحياء

الملخص EN

The periplasm of Gram-negative bacteria is a dynamic and physiologically important subcellular compartment where the constant exposure to potential environmental insults amplifies the need for proper protein folding and modifications.

Top-down proteomics analysis of the periplasmic fraction at the intact protein level provides unrestricted characterization and annotation of the periplasmic proteome, including the post-translational modifications (PTMs) on these proteins.

Here, we used single-dimension ultra-high pressure liquid chromatography coupled with the Fourier transform mass spectrometry (FTMS) to investigate the intact periplasmic proteome of Novosphingobium aromaticivorans.

Our top-down analysis provided the confident identification of 55 proteins in the periplasm and characterized their PTMs including signal peptide removal, N-terminal methionine excision, acetylation, glutathionylation, pyroglutamate, and disulfide bond formation.

This study provides the first experimental evidence for the expression and periplasmic localization of many hypothetical and uncharacterized proteins and the first unrestrictive, large-scale data on PTMs in the bacterial periplasm.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Wu, Si& Brown, Roslyn N.& Payne, Samuel H.& Meng, Da& Zhao, Rui& Tolić, Nikola…[et al.]. 2013. Top-Down Characterization of the Post-Translationally Modified Intact Periplasmic Proteome from the Bacterium Novosphingobium aromaticivorans. International Journal of Proteomics،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-459846

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Wu, Si…[et al.]. Top-Down Characterization of the Post-Translationally Modified Intact Periplasmic Proteome from the Bacterium Novosphingobium aromaticivorans. International Journal of Proteomics No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-459846

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Wu, Si& Brown, Roslyn N.& Payne, Samuel H.& Meng, Da& Zhao, Rui& Tolić, Nikola…[et al.]. Top-Down Characterization of the Post-Translationally Modified Intact Periplasmic Proteome from the Bacterium Novosphingobium aromaticivorans. International Journal of Proteomics. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-459846

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-459846