Cytotoxicity and Modes of Action of the Methanol Extracts of Six Cameroonian Medicinal Plants against Multidrug-Resistant Tumor Cells
المؤلفون المشاركون
Kuete, Victor
Efferth, Thomas
Wiench, Benjamin
Fankam, Aimé G.
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-09-23
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Introduction.
The present study aims at evaluating the cytotoxicity of twelve parts from six Cameroonian medicinal plants on sensitive and drug-resistant cancer cell lines.
We also studied the mode of action of the most active plants, Gladiolus quartinianus, Vepris soyauxii, and Anonidium mannii.
Methods.
The cytotoxicity of the extracts was determined using a resazurin assay.
Flow cytometry was used for cell-cycle analysis and detection of apoptosis, analysis of mitochondrial membrane potential (MMP), and measurement of reactive oxygen species (ROS).
Results.
At 40 g/mL, three extracts showed a growth of CCRF-CEM leukemia cells by less than 50%.
This includes the extracts from G.
quartinianus (GQW; 25.69%), Vepris soyauxii leaves (VSL; 29.82%), and Anonidium mannii leaves (AML; 31.58%).
The lowest IC50 values below 30 μg/mL were obtained with GQW, AML and VSL against 7/9, 8/9, and 9/9 tested cancer cell lines, respectively.
The lowest IC50 values for each plant were 4.09 μg/mL, and 9.14 μg/mL (against U87MG.ΔEGFR cells), respectively, for VSL and AML and 10.57 μg/mL (against CCRF-CEM cells) for GQW.
GQW induced cell cycle arrest between G0/G1 and S phases, whilst VSL and AML induced arrest in G0/G1.
All three extracts induced apoptosis in CCRF-CEM cells by loss of MMP, whilst AML also enhanced production of ROS.
Conclusion.
The three active plants may be a source for the development of new anticancer drugs.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kuete, Victor& Fankam, Aimé G.& Wiench, Benjamin& Efferth, Thomas. 2013. Cytotoxicity and Modes of Action of the Methanol Extracts of Six Cameroonian Medicinal Plants against Multidrug-Resistant Tumor Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-460419
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kuete, Victor…[et al.]. Cytotoxicity and Modes of Action of the Methanol Extracts of Six Cameroonian Medicinal Plants against Multidrug-Resistant Tumor Cells. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-460419
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kuete, Victor& Fankam, Aimé G.& Wiench, Benjamin& Efferth, Thomas. Cytotoxicity and Modes of Action of the Methanol Extracts of Six Cameroonian Medicinal Plants against Multidrug-Resistant Tumor Cells. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-460419
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-460419
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر