Chitosan Feasibility to Retain Retinal Stem Cell Phenotype and Slow Proliferation for Retinal Transplantation
المؤلفون المشاركون
Singh, Amar K.
Casado-Coterillo, Clara
Rodriguez-Crespo, David
Pastor, J. Carlos
Srivastava, Girish K.
Fernandez-Bueno, Ivan
Garcia-Gutierrez, Maria T.
Coronas, Joaquin
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-02-02
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Retinal stem cells (RSCs) are promising in cell replacement strategies for retinal diseases.
RSCs can migrate, differentiate, and integrate into retina.
However, RSCs transplantation needs an adequate support; chitosan membrane (ChM) could be one, which can carry RSCs with high feasibility to support their integration into retina.
RSCs were isolated, evaluated for phenotype, and subsequently grown on sterilized ChM and polystyrene surface for 8 hours, 1, 4, and 11 days for analysing cell adhesion, proliferation, viability, and phenotype.
Isolated RSCs expressed GFAP, PKC, isolectin, recoverin, RPE65, PAX-6, cytokeratin 8/18, and nestin proteins.
They adhered (28 ± 16%, 8 hours) and proliferated (40 ± 20 cells/field, day 1 and 244 ± 100 cells/field, day 4) significantly low (P<0.05) on ChM.
However, they maintained similar viability (>95%) and phenotype (cytokeratin 8/18, PAX6, and nestin proteins expression, day 11) on both surfaces (ChM and polystyrene).
RSCs did not express alpha-SMA protein on both surfaces.
RSCs express proteins belonging to epithelial, glial, and neural cells, confirming that they need further stimulus to reach a final destination of differentiation that could be provided in in vivo condition.
ChM does not alternate RSCs behaviour and therefore can be used as a cell carrier so that slow proliferating RSCs can migrate and integrate into retina.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Srivastava, Girish K.& Rodriguez-Crespo, David& Singh, Amar K.& Casado-Coterillo, Clara& Fernandez-Bueno, Ivan& Garcia-Gutierrez, Maria T.…[et al.]. 2014. Chitosan Feasibility to Retain Retinal Stem Cell Phenotype and Slow Proliferation for Retinal Transplantation. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-460606
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Srivastava, Girish K.…[et al.]. Chitosan Feasibility to Retain Retinal Stem Cell Phenotype and Slow Proliferation for Retinal Transplantation. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-460606
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Srivastava, Girish K.& Rodriguez-Crespo, David& Singh, Amar K.& Casado-Coterillo, Clara& Fernandez-Bueno, Ivan& Garcia-Gutierrez, Maria T.…[et al.]. Chitosan Feasibility to Retain Retinal Stem Cell Phenotype and Slow Proliferation for Retinal Transplantation. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-460606
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-460606
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر