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An Internal Standard-Assisted Synthesis and Degradation Proteomic Approach Reveals the Potential Linkage between VPS4B Depletion and Activation of Fatty Acid β-Oxidation in Breast Cancer Cells
المؤلفون المشاركون
Lin, H. Helen
Liao, Zhongping
Thomas, Stefani N.
Yang, Austin J.
Wan, Yunhu
Ann, David K.
المصدر
International Journal of Proteomics
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-02-04
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الموضوعات
الملخص EN
The endosomal/lysosomal system, in particular the endosomal sorting complexes required for transport (ESCRTs), plays an essential role in regulating the trafficking and destination of endocytosed receptors and their associated signaling molecules.
Recently, we have shown that dysfunction and down-regulation of vacuolar protein sorting 4B (VPS4B), an ESCRT-III associated protein, under hypoxic conditions can lead to the abnormal accumulation of epidermal growth factor receptor (EGFR) and aberrant EGFR signaling in breast cancer.
However, the pathophysiological consequences of VPS4B dysfunction remain largely elusive.
In this study, we used an internal standard-assisted synthesis and degradation mass spectrometry (iSDMS) method, which permits the direct measurement of protein synthesis, degradation and protein dynamic expression, to address the effects of VPS4B dysfunction in altering EGF-mediated protein expression.
Our initial results indicate that VPS4B down-regulation decreases the expression of many proteins involved in glycolytic pathways, while increased the expression of proteins with roles in mitochondrial fatty acid β-oxidation were up-regulated in VPS4B-depleted cells.
This observation is also consistent with our previous finding that hypoxia can induce VPS4B down-regulated, suggesting that the adoption of fatty acid β-oxidation could potentially serve as an alternative energy source and survival mechanism for breast cancer cells in response to hypoxia-mediated VPS4B dysfunction.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liao, Zhongping& Thomas, Stefani N.& Wan, Yunhu& Lin, H. Helen& Ann, David K.& Yang, Austin J.. 2013. An Internal Standard-Assisted Synthesis and Degradation Proteomic Approach Reveals the Potential Linkage between VPS4B Depletion and Activation of Fatty Acid β-Oxidation in Breast Cancer Cells. International Journal of Proteomics،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-460863
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liao, Zhongping…[et al.]. An Internal Standard-Assisted Synthesis and Degradation Proteomic Approach Reveals the Potential Linkage between VPS4B Depletion and Activation of Fatty Acid β-Oxidation in Breast Cancer Cells. International Journal of Proteomics No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-460863
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liao, Zhongping& Thomas, Stefani N.& Wan, Yunhu& Lin, H. Helen& Ann, David K.& Yang, Austin J.. An Internal Standard-Assisted Synthesis and Degradation Proteomic Approach Reveals the Potential Linkage between VPS4B Depletion and Activation of Fatty Acid β-Oxidation in Breast Cancer Cells. International Journal of Proteomics. 2013. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-460863
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-460863
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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