Transforming Growth Factor-β1 Gene Polymorphism (T29C)‎ in Egyptian Patients with Hepatitis B Virus Infection : A Preliminary Study

الملخص EN

The interindividual variations in the capacity of transforming growth factor-β1 (TGF-β1) production have been ascribed to genetic polymorphisms in TGF-β1 gene.

As pathogenesis of HBV has a genetic background, this preliminary study was designed to assess the impact of TGF-β1 (T29C) on the susceptibility of Egyptians to HBV infection.

Genotyping was performed using single stranded polymorphism-polymerase chain reaction (SSP-PCR) in 65 Egyptian hepatitis B patients and 50 healthy controls.

TGF-β1 plasma levels were measured using Enzyme-linked immunosorbent assay (ELISA).

The frequency of CC genotype was significantly higher (P<0.05) in HBV patients compared to controls.

On the contrary, TC genotype did not show significant difference in both groups.

TT genotype was significantly higher (P<0.01) in controls than HBV patients.

Our current preliminary data revealed that the frequency of the genotypes in the controls were within Hardy-Weinberg equilibrium (HWE) while the patients group was out of HWE (P<0.01).

TGF-β1 was significantly (r=-0.684; P<0.001) deceased in the sera of patients as compared to normal subjects.

Depending on our preliminary work, CC genotype may act as a host genetic factor in the susceptibility to HBV infection in Egyptians.

Taken together, the current data pointed to the importance of polymorphism of TGF-β1 gene (T29C) in HBV infection.