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Dual Wavelength RP-HPLC Method for Simultaneous Determination of Two Antispasmodic Drugs : An Application in Pharmaceutical and Human Serum
المؤلفون المشاركون
Hasan, Najmul
Siddiqui, Farhan Ahmed
Khan, Sauleha
Siddiqui, Muhammad Zain
Chaiharn, Mathurot
Sher, Nawab
Khalid, Hira
المصدر
Journal of Analytical Methods in Chemistry
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-10-27
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
A reverse phase stability indicating HPLC method for simultaneous determination of two antispasmodic drugs in pharmaceutical parenteral dosage forms (injectable) and in serum has been developed and validated.
Mobile phase ingredients consist of Acetonitrile : buffer : sulfuric acid 0.1 M (50 : 50 : 0.3 v/v/v), at flow rate 1.0 mL/min using a Hibar μBondapak ODS C18 column monitored at dual wavelength of 266 nm and 205 nm for phloroglucinol and trimethylphloroglucinol, respectively.
The drugs were subjected to stress conditions of hydrolysis (oxidation, base, acid, and thermal degradation).
Oxidation degraded the molecule drastically while there was not so much significant effect of other stress conditions.
The calibration curve was linear with a correlation coefficient of 0.9999 and 0.9992 for PG and TMP, respectively.
The drug recoveries fall in the range of 98.56% and 101.24% with 10 pg/mL and 33 pg/mL limit of detection and limit of quantification for both phloroglucinol and trimethylphloroglucinol.
The method was validated in accordance with ICH guidelines and was applied successfully to quantify the amount of trimethylphloroglucinol and phloroglucinol in bulk, injectable form and physiological fluid.
Forced degradation studies proved the stability indicating abilities of the method.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hasan, Najmul& Chaiharn, Mathurot& Khan, Sauleha& Khalid, Hira& Sher, Nawab& Siddiqui, Farhan Ahmed…[et al.]. 2013. Dual Wavelength RP-HPLC Method for Simultaneous Determination of Two Antispasmodic Drugs : An Application in Pharmaceutical and Human Serum. Journal of Analytical Methods in Chemistry،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-461404
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hasan, Najmul…[et al.]. Dual Wavelength RP-HPLC Method for Simultaneous Determination of Two Antispasmodic Drugs : An Application in Pharmaceutical and Human Serum. Journal of Analytical Methods in Chemistry No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-461404
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hasan, Najmul& Chaiharn, Mathurot& Khan, Sauleha& Khalid, Hira& Sher, Nawab& Siddiqui, Farhan Ahmed…[et al.]. Dual Wavelength RP-HPLC Method for Simultaneous Determination of Two Antispasmodic Drugs : An Application in Pharmaceutical and Human Serum. Journal of Analytical Methods in Chemistry. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-461404
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-461404
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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