Screening of Bacterial Strains for Polygalacturonase Activity : Its Production by Bacillus sphaericus (MTCC 7542)
المؤلفون المشاركون
Gupta, Reena
Shukla, Surendra Kumar
Jayani, Ranveer Singh
المصدر
العدد
المجلد 2010، العدد 2010 (31 ديسمبر/كانون الأول 2010)، ص ص. 1-5، 5ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2010-10-31
دولة النشر
مصر
عدد الصفحات
5
التخصصات الرئيسية
الملخص EN
At present almost all the pectinolytic enzymes used for industrial applications are produced by fungi.
There are a few reports of pectinase production by bacterial strains.
Therefore, in the present study, seventy-four bacterial strains, isolated from soil and rotten vegetable samples, were screened for polygalacturonase production.
The strain PG-31, which gave maximum activity, was identified as Bacillus sphaericus (MTCC 7542).
Maximal quantities of polygalacturonase were produced when a 16-hours-old inoculum was used at 7.5% (v/v) in production medium and incubated in shaking conditions (160 rpm) for 72 hours.
The optimal temperature and pH for bacterial growth and polygalacturonase production were found to be 30∘C and 6.8, respectively.
Maximum enzyme production resulted when citrus pectin was used as the carbon source at a concentration of 1.25% (w/v), whereas other carbon sources led to a decrease (30%–70%) in enzyme production.
Casein hydrolysate and yeast extract used together as organic nitrogen source gave best results, and ammonium chloride was found to be the most suitable inorganic nitrogen source.
The supplementation of media with 0.9% (w/v) D-galacturonic acid led to a 23% increase in activity.
Bacillus sphaericus, a bacterium isolated from soil, produced good amount of polygalacturonase activity at neutral pH; hence, it would be potentially useful to increase the yield of banana, grape, or apple juice.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Jayani, Ranveer Singh& Shukla, Surendra Kumar& Gupta, Reena. 2010. Screening of Bacterial Strains for Polygalacturonase Activity : Its Production by Bacillus sphaericus (MTCC 7542). Enzyme Research،Vol. 2010, no. 2010, pp.1-5.
https://search.emarefa.net/detail/BIM-462083
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Jayani, Ranveer Singh…[et al.]. Screening of Bacterial Strains for Polygalacturonase Activity : Its Production by Bacillus sphaericus (MTCC 7542). Enzyme Research No. 2010 (2010), pp.1-5.
https://search.emarefa.net/detail/BIM-462083
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Jayani, Ranveer Singh& Shukla, Surendra Kumar& Gupta, Reena. Screening of Bacterial Strains for Polygalacturonase Activity : Its Production by Bacillus sphaericus (MTCC 7542). Enzyme Research. 2010. Vol. 2010, no. 2010, pp.1-5.
https://search.emarefa.net/detail/BIM-462083
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-462083
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر