Persistent Amplification of DNA Damage Signal Involved in Replicative Senescence of Normal Human Diploid Fibroblasts
المؤلفون المشاركون
Watanabe, Masami
Yamashita, Shunichi
Suzuki, Keiji
Suzuki, Masatoshi
Kodama, Seiji
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-09-18
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
العلوم الطبيعية والحياتية (متداخلة التخصصات)
الأحياء
الملخص EN
Foci of phosphorylated histone H2AX and ATM are the surrogate markers of DNA double strand breaks.
We previously reported that the residual foci increased their size after irradiation, which amplifies DNA damage signals.
Here, we addressed whether amplification of DNA damage signal is involved in replicative senescence of normal human diploid fibroblasts.
Large phosphorylated H2AX foci (>1.5 μm diameter) were specifically detected in presenescent cells.
The frequency of cells with large foci was well correlated with that of cells positive for senescence-associated β-galactosidase staining.
Hypoxic cell culture condition extended replicative life span of normal human fibroblast, and we found that the formation of large foci delayed in those cells.
Our immuno-FISH analysis revealed that large foci partially localized at telomeres in senescent cells.
Importantly, large foci of phosphorylated H2AX were always colocalized with phosphorylated ATM foci.
Furthermore, Ser15-phosphorylated p53 showed colocalization with the large foci.
Since the treatment of senescent cells with phosphoinositide 3-kinase inhibitor, wortmannin, suppressed p53 phosphorylation, it is suggested that amplification of DNA damage signaling sustains persistent activation of ATM-p53 pathway, which is essential for replicative senescence.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Suzuki, Masatoshi& Suzuki, Keiji& Kodama, Seiji& Yamashita, Shunichi& Watanabe, Masami. 2012. Persistent Amplification of DNA Damage Signal Involved in Replicative Senescence of Normal Human Diploid Fibroblasts. Oxidative Medicine and Cellular Longevity،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-462460
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Suzuki, Masatoshi…[et al.]. Persistent Amplification of DNA Damage Signal Involved in Replicative Senescence of Normal Human Diploid Fibroblasts. Oxidative Medicine and Cellular Longevity No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-462460
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Suzuki, Masatoshi& Suzuki, Keiji& Kodama, Seiji& Yamashita, Shunichi& Watanabe, Masami. Persistent Amplification of DNA Damage Signal Involved in Replicative Senescence of Normal Human Diploid Fibroblasts. Oxidative Medicine and Cellular Longevity. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-462460
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-462460
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر