Antidepressant Fluoxetine Modulates the In Vitro Inhibitory Activity of Buffalo Brain Cystatin : A Thermodynamic Study Using UV and Fluorescence Techniques
المؤلفون المشاركون
المصدر
Biotechnology Research International
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-07-24
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
العلوم الطبية والصيدلة والعلوم الصحية
علم الصيدلة
الموضوعات
الملخص EN
Cystatins constitute a superfamily of homologous proteins.
The major role of cystatins is to regulate the unwanted proteolysis and to protect the organism against endogenous proteases released from lysosomes, invading microorganisms and parasites that use cysteine proteases to enter the body.
Imbalance in regulation of proteolytic activity may lead to a wide range of human diseases.
An enormous progress has been made in understanding of protein degradation process under normal and pathological conditions; infact proteases are now clearly viewed as important drug targets.
Fluoxetine a selective serotonin reuptake inhibitor (SSRI) is an antidepressant.
It is used to treat major depressive disorders.
In the present study binding of fluoxetine to cystatin was studied by UV and fluorescence quenching technique.
Intrinsic fluorescence of fluoxetine complexed with purified buffalo brain cystatin (BC) was measured by selectively exciting the tryptophan residues.
Gradual quenching was observed on complex formation.
When cystatin was added to fluoxetine solutions at a molar ratio of 1 : 0.5, it not only quenched more than half of its fluorescence but also reduced the activity of cystatin.
Stern-Volmer plots obtained from experiments carried out at 25°C showed the quenching of fluorescence to be a collisional phenomenon.
Our results suggest the prime binding site for fluoxetine on BC to be at or near tryptophan residues.
Fluoxetine quenched the fluorescence by a static process, which specifically indicates the formation of a complex.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Amin, Fakhra& Bano, Bilqees. 2014. Antidepressant Fluoxetine Modulates the In Vitro Inhibitory Activity of Buffalo Brain Cystatin : A Thermodynamic Study Using UV and Fluorescence Techniques. Biotechnology Research International،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-463178
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Amin, Fakhra& Bano, Bilqees. Antidepressant Fluoxetine Modulates the In Vitro Inhibitory Activity of Buffalo Brain Cystatin : A Thermodynamic Study Using UV and Fluorescence Techniques. Biotechnology Research International No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-463178
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Amin, Fakhra& Bano, Bilqees. Antidepressant Fluoxetine Modulates the In Vitro Inhibitory Activity of Buffalo Brain Cystatin : A Thermodynamic Study Using UV and Fluorescence Techniques. Biotechnology Research International. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-463178
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-463178
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر